Evaluation of the Anti-East Asian CagA-Specific Antibody for CagA Phenotyping
Evaluation of the Anti-East Asian CagA-Specific Antibody for CagA Phenotyping
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DOI：
10.1128/cvi.00200-09
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发表时间：
2009-11-01
影响因子：
--
通讯作者：
Moriyama, Masatsugu
中科院分区：
文献类型：
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作者：
Lam Tung Nguyen;Uchida, Tomohisa;Moriyama, Masatsugu
cagA基因型的确定通常基于cagA基因的可变3'区的测序。在以前的研究中，我们成功地产生了一个抗东亚CagA特异性抗体（抗EAS抗体）免疫反应，只与东亚CagA，而不是与西方CagA。在少数日本患者中，抗EAS抗体似乎是一个有用的工具，表型CagA免疫化学。本研究的目的是在越南和泰国的大量患者中验证抗EAS Ab免疫组化方法。共招募了385名越南人和泰国人。幽门螺杆菌状态通过三种方法的组合来确定，包括培养、组织学和抗H.幽门螺杆菌抗体根据cagA测序结果作为金标准，计算抗EAS Ab免疫组化方法诊断CagA表型的灵敏度、特异性和准确性。免疫组化方法的敏感性、特异性和准确性分别为96.7%、97.9%和97.1%。此外，抗EAS抗体与非感染胃粘膜无交叉反应。总之，抗EAS抗体免疫组化似乎是一种很好的方法来确定CagA表型。
The determination of the cagA genotype is generally based on sequencing the variable 3' region of the cagA gene. In a previous study, we successfully generated an anti-East Asian CagA-specific antibody (anti-EAS Ab) immunoreactive only with the East Asian CagA and not with the Western CagA. In a small number of Japanese patients, anti-EAS Ab appeared to be a useful tool for phenotyping CagA immunohistochemically. The present study was conducted to validate the anti-EAS Ab immunohistochemistry method in a larger number of patients from Vietnam and Thailand. A total of 385 Vietnamese and Thais were recruited. Helicobacter pylori status was determined by a combination of three methods, including culture, histology, and immunohistochemistry with anti-H. pylori antibody. The sensitivity, specificity, and accuracy of the anti-EAS Ab immunohistochemistry method for the diagnosis of CagA phenotype were calculated based on the results of the cagA sequencing as the gold standard. The sensitivity, specificity, and accuracy of our immunohistochemistry method were 96.7%, 97.9%, and 97.1%, respectively. Moreover, anti-EAS Ab was not cross-reactive with noninfected gastric mucosa. In conclusion, immunohistochemistry with anti-EAS Ab appears to be a good method for determination of CagA phenotype.