Gene expression and functional analyses in brassinosteroid-mediated stress tolerance
Gene expression and functional analyses in brassinosteroid-mediated stress tolerance
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DOI：
10.1111/pbi.12396
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发表时间：
2016-01-01
影响因子：
13.8
通讯作者：
Krishna, Priti
中科院分区：
文献类型：
--
作者：
Divi, Uday K.;Rahman, Tawhidur;Krishna, Priti
油菜素类固醇激素（BR）在植物生长发育中起着重要作用，同时也控制着植物的胁迫反应。BR的这种双重能力从机理的角度来看是令人感兴趣的，并且是在不断变化的气候条件下稳定作物产量的可行解决方案。在这里，我们报告了一个时间过程中的BR响应分析应力和无应力条件下，其结果建立BR包括许多应力相关的功能，即使在无应力条件下，然后伴随着在不利条件下的动态应力响应。BR转录组内发现的是不同的分子标记的两种胁迫激素，脱落酸和茉莉酸，这与增强内源水平的两种激素在BR处理的幼苗。与蛋白质代谢和修饰、防御反应和钙信号传导相关的基因的显著存在突出了它们在BR过程中的相关机制和作用的重要性。对从BR转录组中选出的一组基因的功能缺失突变体进行功能分析，确定了酸性磷酸酶5（ACP 5）、WRKY 33、JACALIN相关凝集素1 -3（JAC-LEC 1 -3）和BR反应受体样激酶（BRRLK）在非生物胁迫中的相关作用。总体而言，本研究的结果提供了BR影响的分子变化与其赋予广泛应激耐受性的能力之间的明确联系，强调了翻译后修饰和蛋白质周转作为BR调节机制的重要性，并证明了BR转录组作为新应激相关调节和结构基因的库。
The plant hormone brassinosteroid (BR) plays essential roles in plant growth and development, while also controlling plant stress responses. This dual ability of BR is intriguing from a mechanistic point of view and as a viable solution for stabilizing crop yields under the changing climatic conditions. Here we report a time course analysis of BR responses under both stress and no-stress conditions, the results of which establish that BR incorporates many stress-related features even under no-stress conditions, which are then accompanied by a dynamic stress response under unfavourable conditions. Found within the BR transcriptome were distinct molecular signatures of two stress hormones, abscisic acid and jasmonic acid, which were correlated with enhanced endogenous levels of the two hormones in BR-treated seedlings. The marked presence of genes related to protein metabolism and modification, defence responses and calcium signalling highlights the significance of their associated mechanisms and roles in BR processes. Functional analysis of loss-of-function mutants of a subset of genes selected from the BR transcriptome identified abiotic stress-related roles for ACID PHOSPHATASE5 (ACP5), WRKY33, JACALIN-RELATED LECTIN1-3 (JAC-LEC1-3) and a BR-RESPONSIVE-RECEPTOR-LIKE KINASE (BRRLK). Overall, the results of this study provide a clear link between the molecular changes impacted by BR and its ability to confer broad-range stress tolerance, emphasize the importance of post-translational modification and protein turnover as BR regulatory mechanisms and demonstrate the BR transcriptome as a repertoire of new stress-related regulatory and structural genes.