Anti arthritic activity of aqueous extract of Indian black tea in experimental and clinical study
Anti arthritic activity of aqueous extract of Indian black tea in experimental and clinical study
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DOI：
10.1007/s13596-012-0087-x
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发表时间：
2012-12-01
影响因子：
--
通讯作者：
Gomes, Antony
中科院分区：
文献类型：
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作者：
Datta, Poulami;Sarkar, Amrita;Gomes, Antony
本研究的目的是评价红茶水提取物对实验性关节炎模型的抗关节炎活性和临床研究。红茶叶（Assam，CTC，India）从经认证的茶供应商处商业收集。用MilliQ水制备BTE，并以干重表示。通过将FCA注射到雄性白化病大鼠的后足的足底表面中，使其产生风湿性关节炎，并将其分为五组：组1（Gr.1）：假手术对照;组2（Gr.2）：关节炎对照;组3（Gr.3）：标准抗关节炎药物吲哚美辛;组4（Gr.4）和组5（Gr.5）：BTE（高剂量和低剂量）。在动物模型中，通过物理、尿液、血清参数和临床血清样品检测BTE的抗关节炎活性。进行关节组织学研究。对类风湿关节炎患者进行BTE治疗，并对血清细胞因子（IL-10、IL-6、IL-4）进行检测。数据表示为平均值+/- SEM（n=6）。对数据进行单因素方差分析（ANOVA），然后使用Graph Pad InStat 3软件进行Tukey检验，以确定统计学显著性。FCA诱导的关节炎大鼠的身体、尿液、血清参数和抗氧化标志物显著改变，但BTE治疗组恢复。关节的组织学研究显示BTE治疗后关节的结构架构恢复。临床研究显示，与关节炎对照组患者相比，BTE治疗组患者的细胞因子水平降低。数据表示为平均值+/- SEM（n=6）。对数据进行单因素方差分析（ANOVA），然后进行Tukey检验以建立统计学显著性（*p
Aim of the study was to evaluate the anti-arthritic activity of aqueous black tea extract against experimental arthritis model and in clinical study. Black tea leave (Assam, CTC, India) was collected commercially from authenticated tea supplier. BTE was prepared with MilliQ water and expressed in terms of dry weight. Rheumatoid arthritis was developed in male albino rats by FCA injection into the sub planter surface of hind foot and divided into five groups: Group-1 (Gr.1): Sham control; Group-2 (Gr.2) : Arthritic control, Group-3 (Gr.3): Standard anti-arthritic drug Indomethacin, Group-4 (Gr.4) & Group-5 (Gr.5) : BTE (high and low dose). Anti-arthritic activity of BTE was examined in animal models through physical, urinary, serum parameters and clinical serum samples. Histological studies of joints were done. Clinical study with BTE performed on the rheumatoid arthritis patients and the serum cytokines (IL-10, IL-6, IL-4) was done. Data was expressed in terms of mean +/- SEM (n=6). The data were subjected to one way analysis of variance (ANOVA) followed by Tukey's test using Graph Pad InStat3 software to establish statistical significance. Physical, urine, serum parameters and anti-oxidant markers, changed significantly in FCA induced arthritic rats but restored in BTE treated groups. Histological studies of joints showed restoration of the structural architecture of the joint after BTE treatment. Clinical study showed decreased cytokines level in the BTE treated group of patients as compared with the arthritis control group of patients. Data was expressed in terms of mean +/- SEM (n=6). The data were subjected to one way analysis of variance (ANOVA) followed by Tukey's test to establish statistical significance (*p