Base recognition of gap sites in DNA-DNA and DNA-RNA duplexes by short oligonucleotides.
Base recognition of gap sites in DNA-DNA and DNA-RNA duplexes by short oligonucleotides.
复制标题
通过短寡核苷酸对 DNA-DNA 和 DNA-RNA 双链体中的间隙位点进行碱基识别。
DOI：
10.1016/j.bmcl.2013.03.054
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sekine M.
中科院分区：
文献类型：
--
作者：
Yamada K;Ohkubo A;Esaka Y;Kanamori T;Masaki Y;Seio K;Sekine M.
为了提高碱基识别能力和灵敏度，我们提出了一个常用的单探针系统的分离寡核苷酸分析成一组三个探针：荧光标记的探针，启动子探针，和短探针。在这项研究中，我们发现，只有4nt的长度的探针可以选择性地结合相应的缺口位点上的复合物组成的目标，荧光标记的探针，和启动子探针，表现出超过14倍的连接之间的匹配和错配序列的差异。此外，我们证明，固定化的短探针准确地识别的差距网站的序列。
To increase base recognition capability and sensitivity, we propose the separation of a commonly used single-probe system for oligonucleotide analysis into a set of three probes: a fluorophore-labeled probe, a promoter probe, and a short probe. In this study, we found that the probes of only 4nt in length can selectively bind the corresponding gap site on complexes consisting of the target, fluorophore-labeled probe, and promoter probe, exhibiting a more than 14-fold difference in ligation between the matched and mismatched sequences. Moreover, we demonstrated that the immobilized short probes accurately recognized the sequences of the gap sites.