Experimental intracerebral hemorrhage: effect of lysed erythrocytes on brain edema and blood-brain barrier permeability.
Experimental intracerebral hemorrhage: effect of lysed erythrocytes on brain edema and blood-brain barrier permeability.
复制标题
实验性脑出血：裂解红细胞对脑水肿和血脑屏障通透性的影响。
DOI：
10.1007/978-3-7091-6738-0_65
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Hoff,JT
中科院分区：
文献类型：
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作者：
Bhasin,RR;Xi,G;Hua,Y;Keep,RF;Hoff,JT
了解脑出血（ICH）后水肿形成的机制对于制定这种情况的治疗方案非常重要。本研究探讨了红细胞（RBC）溶解和血红蛋白在脑出血后水肿形成中的作用。输注裂解RBC的大鼠在24小时后出现显著脑水肿，这与血脑屏障（BBB）对α-氨基异丁酸的渗透性增加3倍有关。我们以前已经证明，脑内注射浓缩红细胞不会在一天内引起水肿形成，但会在三天内引起。在这项研究中，我们发现，包装红细胞没有造成显着的血脑屏障破坏在一天，但产生了4倍的增加血脑屏障通透性在三天。这些研究表明，ICH后，红细胞内物质的泄漏，细胞溶解促进，导致迟发性水肿。这通过实验诱导的裂解和自然发生的体内裂解两者证实。
Understanding the mechanism of edema formation following intracerebral hemorrhage (ICH) is important for developing treatment protocols for this condition. This study examines the role of red blood cell (RBC) lysis and hemoglobin in edema formation following ICH. Significant brain edema developed after 24 hours in rats infused with lysed RBCs and this was associated with a 3-fold increase blood brain barrier (BBB) permeability to α-aminoisobutyric acid. We have previously shown that intracerebral injection of packed RBCs does not cause edema formation at one day but does at three days. In this study, we found that packed RBCs did not cause significant BBB disruption at one day but produced a 4-fold increase in BBB permeability at three days. These studies show that following ICH the leakage of substances inside the RBC, facilitated by cellular lysis, results in delayed edema. This was confirmed by both experimentally induced lysis and naturally occurring in-vivo lysis.