Invertebrate cytokines: tunicate cell proliferation stimulated by an interleukin 1-like molecule.
Invertebrate cytokines: tunicate cell proliferation stimulated by an interleukin 1-like molecule.
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无脊椎动物细胞因子：被白细胞介素 1 样分子刺激的被囊细胞增殖。
DOI：
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发表时间：
1991
影响因子：
11.1
通讯作者：
G. Beck
中科院分区：
文献类型：
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作者：
D. Raftos;E. Cooper;G. Habicht;G. Beck
被囊类咽细胞包括淋巴细胞样细胞和颗粒变形细胞。它们参与被囊类动物特定的同种异体和吞噬反应。人们对它们的监管或控制知之甚少。被囊动物白细胞介素 1 (IL-1) 样成分可在体外刺激这些细胞的增殖。该级分被命名为被囊动物 IL-1 β，通过凝胶过滤和色谱聚焦色谱从被囊动物血淋巴中分离出来。对被囊动物 IL-1 β 的促有丝分裂反应是剂量依赖性的，并且可以通过从培养基中去除被囊动物 IL-1 β 来快速消除。第二个被囊类血淋巴组分对被囊类细胞增殖没有影响，尽管它在小鼠胸腺细胞增殖测定中表现出类似 IL-1 的活性。植物血凝素与任一部分均不产生协同作用。这些数据根据无脊椎动物中 IL-1 样分子的功能和进化进行了讨论。
Tunicate pharyngeal cells include lymphocyte-like cells and granular amoebocytes. They are involved in the specific allogeneic and phagocytic reactions of tunicates. Little is known about their regulation or control. A tunicate interleukin 1 (IL-1)-like fraction is shown to stimulate the proliferation of these cells in vitro. This fraction, designated tunicate IL-1 beta, was isolated from tunicate hemolymph by gel filtration and chromatofocusing chromatography. Mitogenic responses to tunicate IL-1 beta were dose dependent and could be eliminated rapidly by removing tunicate IL-1 beta from culture medium. A second tunicate hemolymph fraction had no effect on tunicate cell proliferation even though it exhibited IL-1-like activity in a mouse thymocyte proliferation assay. Phytohemagglutin did not act synergistically with either fraction. These data are discussed in terms of the function and evolution of IL-1-like molecules in invertebrates.