MOLECULAR-CLONING OF PHENYLALANINE AMMONIA-LYASE CDNA AND CLASSIFICATION OF VARIETIES AND CULTIVARS OF TEA PLANTS (CAMELLIA-SINENSIS) USING THE TEA PAL CDNA PROBE
MOLECULAR-CLONING OF PHENYLALANINE AMMONIA-LYASE CDNA AND CLASSIFICATION OF VARIETIES AND CULTIVARS OF TEA PLANTS (CAMELLIA-SINENSIS) USING THE TEA PAL CDNA PROBE
复制标题
DOI：
10.1007/bf00223703
复制
发表时间：
1994-11-01
影响因子：
5.4
通讯作者：
KONDO, S
中科院分区：
文献类型：
--
作者：
MATSUMOTO, S;TAKEUCHI, A;KONDO, S
使用标记的水稻 PAL cDNA 作为探针克隆茶 (Camellia sinensis) 苯丙氨酸解氨酶 (PAL) cDNA。使用茶 PAL cDNA 通过限制性片段长度多态性 (RFLP) 分析研究茶树的 PAL 基因。茶树的 PAL 遗传变异比预测的要大得多，因为阿萨姆杂种中存在各种杂交片段，这些杂种是 C. sinensis var 'assamica' 和 var 'sinensis' 之间的杂种。另一方面，属于 var 'sinensis' 的日本绿茶品种的杂交带型可分为 5 个类群。此外，短长的PAL探针，包括3'非翻译序列，大约280 bp，检测到3个不同长度的DNA片段，分别命名为A、B和D。PAL基因遗传追踪实验表明，A、B和D片段是按照孟德尔单基因比例遗传的。因此，A、B和D片段可识别的PAL基因是多个等位基因，并且PAL基因在茶单倍体基因组中作为单个基因存在。还清楚的是，五组日本绿茶品种的特征在于这些 PAL 片段的组成。通过使用茶 PAL cDNA 的 RFLP 分析，我们成功地区分了儿茶素含量高和低的阿萨姆杂种和日本绿茶品种，并在品种水平上对日本绿茶进行了分组。
Tea (Camellia sinensis) phenylalanine ammonia-lyase (PAL) cDNA was cloned using labelled rice PAL cDNA as a probe. The PAL genes of the tea plant were investigated by restriction fragment length polymorphism (RFLP) analysis using tea PAL cDNA. PAL genetic variation in tea plants was much larger than predicted due to the presence of various hybridized fragments in the Assam hybrids, which are hybrids between C. sinensis var 'assamica' and var 'sinensis'. On the other hand, hybridized band patterns of Japanese green tea cultivars belonging to var 'sinensis' could be divided into five groups. Furthermore, a short-length PAL probe, about 280 bp including the 3' untranslated sequence, detected 3 DNA fragments of different lengths, which were named A, B and D. An experiment tracing the PAL gene heredity showed that A, B and D fragments were inherited according to the Mendelian monogenic ratio. Therefore, PAL genes identifiable by A, B and D fragments are multiple alleles, and the PAL gene is present as a single gene in the tea haploid genome. It was also clear that five groups of Japanese green tea cultivars were characterized by the composition of these PAL fragments. From RFLP analysis using tea PAL cDNA, we succeeded in distinguishing Assam hybrids and Japanese green tea cultivars with high and low catechin content, respectively, and in grouping Japanese green tea at the cultivar level.