REPRESSION AND TURNOVER PATTERN FUSHI-TARAZU RNA IN THE EARLY DROSOPHILA EMBRYO
REPRESSION AND TURNOVER PATTERN FUSHI-TARAZU RNA IN THE EARLY DROSOPHILA EMBRYO
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DOI：
10.1016/0092-8674(86)90517-9
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发表时间：
1986-12-05
期刊：
影响因子：
64.5
通讯作者：
KORNBERG, T
中科院分区：
文献类型：
--
作者：
EDGAR, BA;WEIR, MP;KORNBERG, T
果蝇基因fushi tarazu（ftz）的转录本在胚胎中的表达经历了一系列的空间模式，在细胞胚盘阶段以7条带的模式达到顶峰。我们使用RNA合成抑制剂（α-RNA）研究了这种模式的产生。鹅膏蕈碱）和蛋白质合成（放线菌酮）。注射α-鹅膏蕈碱揭示了ftz RNA在胚胎中的转换非常迅速，我们认为这可能是影响ftz RNA模式快速变化的关键。注射放线菌酮后，ftz表达的正常区域增加，产生了依赖于注射时间的新的表达模式。这些新的模式表明，两个叠加的抑制系统建立了正常的，七带模式的ftz表达。一个系统在胚胎的整个长度上建立一个带状模式，另一个系统将实际表达限制在胚胎的中间部分。
Embryonic expression of transcripts from the Drosophila gene fushi tarazu (ftz) progresses through a series of spatial patterns, culminating in a seven-banded pattern at the cellular blastoderm stage. We studied the generation of this pattern using inhibitors of RNA synthesis (.alpha.-amanitin) and protein synthesis (cycloheximide). Injections of .alpha.-amanitin revealed that ftz RNA turns over extremely rapidly in the embryo, and we think that this may be essential to effect rapid changes in ftz RNA patterns. Injections of cycloheximide added to the normal domains of ftz expression, creating novel expression patterns that were dependent on the time of injection. These novel patterns suggest that two superimposed systems of repression establish the normal, seven-banded pattern of ftz expression. One system sets up a banded pattern over the entire length of the embryo, and the other restricts actual expression to the middle portion of the embryo.