Organization in lipid membranes containing cholesterol
Organization in lipid membranes containing cholesterol
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DOI：
10.1103/physrevlett.89.268101
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发表时间：
2002-12-23
影响因子：
8.6
通讯作者：
Keller, SL
中科院分区：
文献类型：
--
作者：
Veatch, SL;Keller, SL
细胞膜中筏形成的一个基本属性是脂质横向分离成共存的液相。使用荧光显微镜，我们观察到自发的横向分离在自由浮动的巨大单层囊泡。我们记录共存的液体域的组成和温度范围显着比以前报道的更广泛。此外，我们还建立了双层和单层的可渗透性之间的相关性。例如，在双层膜中产生液体域的相同脂质混合物在单层的相图中产生两个上相容性临界点。
A fundamental attribute of raft formation in cell membranes is lateral separation of lipids into coexisting liquid phases. Using fluorescence microscopy, we observe spontaneous lateral separation in free-floating giant unilamellar vesicles. We record coexisting liquid domains over a range of composition and temperature significantly wider than previously reported. Furthermore, we establish correlations between miscibility in bilayers and in monolayers. For example, the same lipid mixtures that produce liquid domains in bilayer membranes produce two upper miscibility critical points in the phase diagrams of monolayers.