Nitrate nutrition and temperature effects on wheat: soluble components of leaves and carbon fluxes to amino acids and sucrose
Nitrate nutrition and temperature effects on wheat: soluble components of leaves and carbon fluxes to amino acids and sucrose
复制标题
硝酸盐营养和温度对小麦的影响：叶子的可溶性成分以及氨基酸和蔗糖的碳通量
DOI：
10.1093/jxb/38.7.1091
复制
发表时间：
1987
影响因子：
6.9
通讯作者：
A. Keys
中科院分区：
文献类型：
--
作者：
D. Lawlor;F. Boyle;A. T. Young;A. Kendall;A. Keys
Lawlor， D. W., Boyle， F. A., Young， A. T., Kendall， A. C.和Keys， A. J. 1987。硝酸盐营养和温度对小麦的影响：叶片可溶性组分和对氨基酸和蔗糖的碳通量。exp. Bot. 38: 1091-1103。在两种温度下以两种硝酸盐供应速率生长的小麦的第三片叶片中，测量了一些代谢物的数量和光合产物中14co2的掺入量，以分析环境条件对碳通量的影响。单位面积四磷酸核酮糖和3-磷酸甘油酸含量在缺硝条件下较高，随叶龄降低，但与温度差异不一致。在低温和低硝酸盐条件下，幼叶蔗糖含量高于温暖条件，且随年龄的增长而下降，各处理的蔗糖含量相近。淀粉随叶龄积累，在低温条件下比在温暖条件下略多，硝酸盐缺乏时。在低温条件下，添加硝酸盐的叶片谷氨酸（加谷氨酰胺）、天冬氨酸（加天冬酰胺）、甘氨酸和丝氨酸含量最高；讨论了随叶龄和条件的变化。在稳定条件下（在20°C下长达10分钟）暴露于14co2的植物，在温暖温度下生长的同化产物的14C含量略高于在凉爽温度下生长并添加硝酸盐的植物。添加硝酸盐增加了氨基酸（尤其是丝氨酸和甘氨酸）中14C的比例和碳对氨基酸的通量，降低了磷酸糖和蔗糖中的14C比例。低温提高了氨基酸（主要是甘氨酸和丝氨酸）中14C的比例，降低了蔗糖中的14C比例。氨基酸和碳水化合物之间碳通量平衡的变化与乙醇酸途径代谢和氨基酸合成的替代途径有关。关键词：小麦，温度，硝酸盐供应，碳通量，蔗糖，氨基酸。通讯单位：赫茨哈彭登洛桑试验站生理与环境物理系。al52jq，英国
Lawlor, D. W., Boyle, F. A., Young, A. T., Kendall, A. C. and Keys, A. J. 1987. Nitrate nutrition and temperature effects on wheat: Soluble components of leaves and carbon fluxes to amino acids and sucrose.—J. exp. Bot. 38: 1091-1103. Amounts of some metabolites and the incorporation of 14C02 into photosynthetic products were measured in the third leaf of wheat, grown with two rates of nitrate supply at two temperatures, to analyse the effects of environmental conditions on the fluxes of carbon. Ribulose tephosphate and 3-phosphoglyceric acid content per unit area were greater under nitrate deficiency and decreased with leaf age, but did not differ consistently with temperature. Sucrose content of young leaves was larger in cool than in warm conditions and with low nitrate, and decreased with age to similar values in all treatments. Starch accumulated with leaf age, slightly more in cool than warm conditions, and with nitrate deficiency. Glutamate (plus glutamine), aspartate (plus asparagine), glycine and serine content of leaves were greatest with added nitrate in cool temperature; changes with leaf age and conditions are discussed. The 14C content of assimilation products after exposure to 14C02 (for up to 10 min at 20 °C) under steady-state conditions was slightly greater in plants grown in the warm than in the cool temperature and with additional nitrate. Additional nitrate increased the proportion of 14C in, and flux of carbon to, amino acids, particularly serine and glycine, and decreased it in sugar phosphates and sucrose. Cool growth temperatures increased the proportion of 14C in amino acids (pre dominantly glycine and serine) and decreased that in sucrose. Changes in the balance of carbon fluxes between amino acids and carbohydrates are discussed in relation to glycolate pathway metabolism and alternative routes of amino acid synthesis. Key words— Wheat, temperature, nitrate supply, carbon flux, sucrose, amino acids. Correspondence to: Physiology and Environmental Physics Department, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ, U.K.