Dietary vitamin D(3) deprivation suppresses fibroblast growth factor 23 signals by reducing serum phosphorus levels in laying hens.
Dietary vitamin D(3) deprivation suppresses fibroblast growth factor 23 signals by reducing serum phosphorus levels in laying hens.
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膳食维生素 D3 剥夺通过降低蛋鸡血清磷水平来抑制成纤维细胞生长因子 23 信号
DOI：
10.1016/j.aninu.2021.07.010
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发表时间：
2022-06
期刊：
影响因子：
6.3
通讯作者：
Ren, Zhouzheng
中科院分区：
文献类型：
--
作者：
Yan, Jiakun;Pan, Chong;Liu, Yanli;Liao, Xujie;Chen, Jionghao;Zhu, Yufei;Huang, Xinhuo;Yang, Xiaojun;Ren, Zhouzheng
关键词：
本研究旨在探讨饲粮中添加维生素D3对产蛋鸡成纤维细胞生长因子23（FGF 23）信号及磷稳态代谢的影响。试验选用40周龄海兰褐蛋鸡14只，随机分为2组：1）维生素D3限制组（n = 7），饲喂0 IU/kg维生素D3日粮; 2）维生素D3常规组（n = 7），饲喂1,600 IU/kg维生素D3日粮。试验持续21 d。测定血清参数、磷和钙排泄状态以及II型钠-磷酸盐共转运蛋白（NPt 2）、FGF 23信号和维生素D3代谢调节剂的组织表达。饲喂维生素D3限制饮食的母鸡血清磷水平降低（31.3%，P = 0.028），与饲喂常规维生素D3饮食的母鸡相比。在低磷条件下，维生素D3限制蛋鸡表现为：1）肾脏25-羟维生素D1-α-羟化酶表达降低（CYP 27 B1，52.8%，P = 0.036）和1，25-二羟维生素D 24-羟化酶（CYP 24 A1，99.4%，P = 0.032）; 2）抑制血清FGF 23水平（14.6%，P = 0.048），血清碱性磷酸酶水平升高（414.1%，P = 0.012）; 3）成纤维细胞生长因子受体mRNA表达降低（FGFR 1，提高85.2%，P = 0.003，FGFR 2，提高89.4%，P = 0.014，FGFR 3，提高88.8%，P = 0.017，FGFR 4，提高89.6%，P = 0.030）; 4）肾组织FGFR 1 mRNA表达降低（增加65.5%，P = 0.021），FGFR 4（66.0%，P = 0.050）和KLOTHO（68.8%，P = 0.038）; 5）肾脏2a型钠磷共转运蛋白表达减少（54.3%，P = 0.039）;和6）增加排泄钙百分比（26.9%，P = 0.002）。总之，饲粮维生素D3的剥夺通过降低血清FGF 23水平和抑制颅骨和肾脏FGF 23受体mRNA的表达来降低蛋鸡的FGF 23信号。
The present study was carried out to evaluate the effect of dietary supplemental vitamin D3 on fibroblast growth factor 23 (FGF23) signals as well as phosphorus homeostasis and metabolism in laying hens. Fourteen 40-week-old Hy-Line Brown layers were randomly assigned into 2 treatments: 1) vitamin D3 restriction group (n = 7) fed 0 IU/kg vitamin D3 diet, and 2) regular vitamin D3 group (n = 7) fed 1,600 IU/kg vitamin D3 diet. The study lasted for 21 d. Serum parameters, phosphorus and calcium excretion status, and tissue expressions of type II sodium-phosphate co-transporters (NPt2), FGF23 signals and vitamin D3 metabolic regulators were determined. Hens fed the vitamin D3 restricted diet had decreased serum phosphorus levels (by 31.3%, P = 0.028) when compared to those fed regular vitamin D3 diet. In response to the decreased serum phosphorus, the vitamin D3 restricted laying hens exhibited: 1) suppressed kidney expressions of 25-hydroxyvitamin D 1-α-hydroxylase (CYP27B1, by 52.8%, P = 0.036) and 1,25-dihydroxyvitamin D 24-hydroxylase (CYP24A1, by 99.4%, P = 0.032); 2) suppressed serum levels of FGF23 (by 14.6%, P = 0.048) and increased serum alkaline phosphatase level (by 414.1%, P = 0.012); 3) decreased calvaria mRNA expressions of fibroblast growth factor receptors (FGFR1, by 85.2%, P = 0.003, FGFR2, by 89.4%, P = 0.014, FGFR3, by 88.8%, P = 0.017, FGFR4, by 89.6%, P = 0.030); 4) decreased kidney mRNA expressions of FGFR1 (by 65.5%, P = 0.021), FGFR4 (by 66.0%, P = 0.050) and KLOTHO (by 68.8%, P = 0.038); 5) decreased kidney protein expression of type 2a sodium-phosphorus co-transporters (by 54.3%, P = 0.039); and 6) increased percent excreta calcium (by 26.9%, P = 0.002). In conclusion, the deprivation of dietary vitamin D3 decreased FGF23 signals in laying hens by reducing serum FGF23 level and suppressing calvaria and kidney mRNA expressions of FGF23 receptors.
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影响因子：
5.5
作者：
Hernando, Nati;Pastor-Arroyo, Eva Maria;Wagner, Carsten A.
通讯作者：
Wagner, Carsten A.
DOI：
10.1152/ajpgi.00243.2005
发表时间：
2005-12-01
影响因子：
4.5
作者：
Kolek, OI;Hines, ER;Ghishan, FK
通讯作者：
Ghishan, FK
影响因子：
3.7
作者：
Han X;Yang J;Li L;Huang J;King G;Quarles LD
通讯作者：
Quarles LD
影响因子：
6.2
作者：
Fujiwara, I;Aravindan, R;Drezner, MK
通讯作者：
Drezner, MK
影响因子：
6.2
作者：
Hernando, Nati;Myakala, Komuraiah;Biber, Juerg
通讯作者：
Biber, Juerg