Culturing partial denitrification biofilm in side stream incubator with ordinary activated sludge as inoculum: One step closer to mainstream Anammox upgrade
Culturing partial denitrification biofilm in side stream incubator with ordinary activated sludge as inoculum: One step closer to mainstream Anammox upgrade
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以普通活性污泥为接种物在侧流培养箱中培养部分反硝化生物膜：距离主流厌氧氨氧化升级又近了一步
DOI：
10.1016/j.biortech.2022.126679
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发表时间：
2022
影响因子：
11.4
通讯作者：
Xiaoxia Wang
中科院分区：
文献类型：
--
作者：
Xincheng Zhang;Yuanyuan Miao;Deshuang Yu;Yanling Qiu;Ji Zhao;Xiaoxia Wang
最近提出了在厌氧区添加载体的主流厌氧氨氧化升级方法，它依靠负责部分反硝化(PD)的反硝化菌来产生厌氧氨氧化细菌必需的亚硝酸盐。尽管如此，它们在自然形成的生物膜中的低丰度导致亚硝酸盐供应不足。本研究对钯生物膜的连续培养进行了研究。通过接种普通活性污泥，在54d内迅速建立了PD工艺。在此期间，按顺序减少碳氮比和缺氧时间可能是有效的策略。添加载体改变了微生物群落，尤其是黄杆菌的增殖。单独使用成熟的Pd生物膜时，硝酸盐到亚硝酸盐的转化率很高(>；70%)。与此同时，硝酸盐还原和亚硝酸盐的生成过程都放缓了，持续了∼90分钟。此外，在生物膜中还检测到大量的简单吡喃二酸根离子。这项研究还表明，定期从侧流孵化器中收集PD相关功能细菌可以促进主流厌氧氨氧化的升级。
Recently, adding carriers into anoxic zone is proposed for mainstream Anammox upgrade, which relied on the denitrifiers responsible for partial denitrification (PD) to generate essential nitrite for Anammox bacteria. Still, their low abundance in the naturally formed biofilm leads to insufficient nitrite supply. This study investigated the sequential culturing of PD biofilm. By inoculating ordinary activated sludge, the PD process was quickly established within 54-day. During that, decreasing carbon to nitrogen ratio and anoxic duration in order might be effective strategies. Adding carriers shifted the microbial community, especially the proliferation ofFlavobacterium. When solely using the mature PD biofilm, high nitrate to nitrite transformation ratio (>70%) was obtained. Meanwhile, both nitrate-reducing and nitrite-generating processes slowed down and lasted ∼90 min. In addition, abundantSimplicispiracandidate for PD was detected in biofilm. This study also suggests that regularly harvesting PD-related functional bacteria from a side-stream incubator promotes mainstream Anammox upgrade.