Maternal transfer of complement components C3-1, C3-3, C3-4, C4, C5, C7, Bf, and Df to offspring in rainbow trout (Oncorhynchus mykiss)
Maternal transfer of complement components C3-1, C3-3, C3-4, C4, C5, C7, Bf, and Df to offspring in rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1007/s00251-006-0096-3
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发表时间：
2006-04-01
期刊：
影响因子：
3.2
通讯作者：
Dalmo, RA
中科院分区：
文献类型：
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作者：
Lvoll, M;Kilvik, T;Dalmo, RA
发育中鱼类的防御机制知之甚少，但在淋巴器官和免疫能力成熟之前，先天机制是必不可少的。补体系统代表了先天免疫的主要部分。我们的主要目标是绘制鱼类发育早期补体成分的存在。虹鳟鱼卵，胚胎，和孵化的C3-1，C3-3，C3-4，C4，C5，C7，因子B和因子D转录的发病和持续时间进行了测定，使用实时逆转录-聚合酶链反应。在一般情况下，补体转录水平稳步上升，从受精后第28天孵化，随后在卵黄囊吸收下降。所有研究的补体蛋白都是在未受精的卵子中发现的。在整个研究期间，转录本和蛋白质水平之间没有相关性。补体蛋白在第7 - 35天出现在肝脏、肾脏和肠中，但直到第77天才出现在心脏中。这项研究是第一个解决几个补体成分的个体发育，并代表了第一个证据表明，母亲转移的补体成分，而不是C3，发生在硬骨鱼。
Defense mechanisms in developing fish are poorly known but before maturation of lymphoid organs and immunocompetence, innate mechanisms are essential. The complement system represents a major part of innate immunity. Our main objective was to map the presence of complement components early in fish development. Rainbow trout eggs, embryos, and hatchlings were assayed for the onset and duration of C3-1, C3-3, C3-4, C4, C5, C7, factor B, and factor D transcription using real-time reverse transcription-polymerase chain reaction. In general, complement transcript levels increased steadily from day 28 postfertilization to hatch, followed by a decrease during yolk-sac resorption. All the complement proteins studied were found in unfertilized eggs. There was no correlation between the transcript and protein levels throughout the study period. Complement proteins appeared in the liver, kidney, and intestine between day 7 and 35 but not until day 77 in the heart. This study is the first to address the ontogeny of several complement components and represents the first evidence that maternal transfer of complement components, other than C3, occurs in teleost fish.