Identification of proteins associated with the production of melanin and with pathogenicity in maize pathogen Curvularia lunata
Identification of proteins associated with the production of melanin and with pathogenicity in maize pathogen Curvularia lunata
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DOI：
10.1007/s13313-015-0382-3
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发表时间：
2015-10
影响因子：
1.4
通讯作者：
Jin-xin Gao;Jing Jing-Jing;Tong Liu;Chuan-jin Yu;Yaqian Li;Jie Chen
中科院分区：
文献类型：
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作者：
Jin-xin Gao;Jing Jing-Jing;Tong Liu;Chuan-jin Yu;Yaqian Li;Jie Chen
新月弯孢菌产生1，8-二羟基萘（DHN）黑色素。在此之前，我们已经成功地克隆并沉默了Brn 1基因，该基因参与DNH黑色素的产生和致病性。然而，我们假设其他蛋白质也可能与黑色素的产生或致病性有关。本研究采用双向凝胶电泳和质谱技术相结合的蛋白质组学方法，对与C. lunata野生型CX-3和Brn 1沉默突变体T5。在T5中成功鉴定了一个上调和七个下调的蛋白。转醛醇酶、NADP依赖性甘露醇脱氢酶、甲酸脱氢酶和UTP-葡萄糖-1-磷酸尿苷酰转移酶参与能量代谢，延伸因子3促进黑色素和应激耐受相关蛋白（HSP 30和HSP 70）的合成，过氧化氢酶（CAT）参与C. lunata。在这些蛋白质中，CAT作为感兴趣的蛋白质被进一步分析。生物信息学分析表明，CAT由748个氨基酸组成，在系统发育树中与盾壳菌CAT同源物聚为一个分支。关于CAT基因的确切作用的进一步研究正在进行中。
Curvularia lunata produces 1,8-dihydroxynaphthalene (DHN) melanin. Previously, we have successfully cloned and silenced the Brn1 gene, which is involved in DNH melanin production and pathogenicity. However, we hypothesise that other proteins may also be related to melanin production or pathogenicity. In this study, a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry techniques was used to identify the specific proteins associated with Brn1 expression in C. lunata wild-type CX-3 and Brn1-silenced mutant T5. One up-regulated and seven down-regulated proteins were successfully identified in T5. Whilst transaldolase, NADP-dependent mannitol dehydrogenase, formate dehydrogenase, and UTP-glucose-1-phosphate uridylyltransferase are involved in energy metabolism, elongation factor 3 facilitates the synthesis of important proteins required for the production of melanin and stress tolerance-related proteins (HSP30 and HSP70) and catalase (CAT) contribute to the pathogenicity of C. lunata. Of these proteins, CAT was further analysed as a protein of interest. Bioinformatics analysis showed that CAT consists of 748 amino acids and clustered into one branch with Dothideomycete CAT homologues in a phylogenetic tree. Further research on the exact role of the CAT gene is underway.