INVIVO REGIONAL LEVELS OF PGE AND THROMBOXANE IN MOUSE-BRAIN - EFFECT OF DECAPITATION, FOCUSED MICROWAVE FIXATION, AND INDOMETHACIN
INVIVO REGIONAL LEVELS OF PGE AND THROMBOXANE IN MOUSE-BRAIN - EFFECT OF DECAPITATION, FOCUSED MICROWAVE FIXATION, AND INDOMETHACIN
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DOI：
10.1016/0090-6980(83)90177-6
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
RANDALL, CL
中科院分区：
文献类型：
--
作者：
ANTON, RF;WALLIS, C;RANDALL, CL
用聚焦微波固定技术测定小鼠脑内前列腺素E和血栓素B2的基础水平。聚焦微波(3.5KW/0.4KW S)照射C3H小鼠头部产生的PGE和TXB_2的基础值比断头处死的小鼠低5倍。吲哚美辛(10 mg/kg)可阻断断头后PGE和TXB_2水平的升高，并与聚焦微波辐射相似。吲哚美辛预处理联合微波固定对PG水平的降低作用不比单独微波处理更明显。当使用微波固定时，局部(大脑皮层、全小脑、中脑、下丘脑)PGE和TXB_2水平均无差异。各地区PGE水平均显著高于TXB_2。断头后TXB_2较PGE升高更明显。小脑在断头后产生的PGE和TXB_2比其他区域少。证实了聚焦微波辐射技术在体内检测小鼠脑内基础PG水平的有效性。
A focused microwave fixation technique was tested for use in determining basal PG[prostaglandin]E and thromboxane [TX] B2 levels of mouse brain. Focused microwave irradiation (3.5 KW/0.4 s) to the head of C3H mice produced basal values of PGE and TXB2 which were 5-fold less than those in animals killed by decapitation. Indomethacin (10 mg/kg) pretreatment blocked the decapitation rise in PGE and TXB2 levels and gave values similar to focused microwave irradiation. Indomethacin pretreatment combined with microwave fixation did not reduce PG levels more than microwave treatment alone. When microwave fixation was used, there was no difference in regional (cerebral cortex, whole cerebellum, midbrain, hypothalamus) levels of either PGE or TXB2. PGE levels were significantly higher than TXB2 in all regions. After decapitation there was a greater increase in TXB2 than PGE. The cerebellum produced less PGE and TXB2 after decapitation compared to the other regions. The usefulness of the focused microwave irradiation technique for examining in vivo basal PG levels in mouse brain was confirmed.