Brain-derived neurotrophic factor prevents the endothelial barrier dysfunction induced by interleukin-1β and tumor necrosis factor-α
Brain-derived neurotrophic factor prevents the endothelial barrier dysfunction induced by interleukin-1β and tumor necrosis factor-α
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DOI：
10.1111/jre.12226
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发表时间：
2015-08-01
影响因子：
3.5
通讯作者：
Kurihara, H.
中科院分区：
文献类型：
--
作者：
Matsuda, S.;Fujita, T.;Kurihara, H.
背景和目的脑源性神经营养因子（BDNF）促进牙周组织再生。尽管需要局部炎症步骤来启动随后的组织再生过程，但过度的炎症可能会抑制或延迟组织再生。因此，炎症的调节对于牙周组织再生至关重要。在本研究中，我们检测了 BDNF 对白细胞介素 (IL)-1 或肿瘤坏死因子 (TNF) 诱导的人微血管内皮细胞 (HMVEC) 屏障功能障碍的影响，以确定 BDNF 对牙周组织再生中局部炎症调节的影响。 使用 Transwell 板进行葡聚糖转运测定。通过免疫印迹和免疫荧光显微镜检测血管内皮（VE）钙粘蛋白的表达。结果BDNF（25ng/mL）抑制IL-1和TNF-诱导的内皮通透性增加。 IL-1 和 TNF- 降低了 VE-钙粘蛋白水平，而 BDNF 恢复了 HMVEC 的减少。 BDNF 通过 TrkB 保护 IL-1 和 TNF-α 诱导的内皮通透性增加。培养液中单独添加BDNF可增加HMVEC中VE-cadherin的表达。结论BDNF在抑制内皮屏障功能障碍中发挥重要作用，这表明它可能有助于增强牙周组织再生。
Background and ObjectiveBrain-derived neurotrophic factor (BDNF) promotes the regeneration of periodontal tissue. Although a local inflammatory step is required to initiate the subsequent process of tissue regeneration, excessive inflammation may inhibit or delay tissue regeneration. Therefore, the regulation of inflammation is essential for periodontal tissue regeneration. In the present study, we examined the influence of BDNF on the human microvascular endothelial cell (HMVEC) barrier dysfunction induced by interleukin (IL)-1 or tumor necrosis factor (TNF)- to determine the effects of BDNF on the regulation of local inflammation in periodontal tissue regeneration.Material and MethodsEndothelial permeability was analyzed using a Dextran transport assay with transwell plates. The expression of vascular endothelial (VE)-cadherin was assessed by immunoblotting and immunofluorescence microscopy.ResultsBDNF (25ng/mL) inhibited increase induced in endothelial permeability by IL-1 and TNF-. IL-1 and TNF- decreased VE-cadherin protein levels, while BDNF recovered the reduction in HMVECs. BDNF protected the increase induced in endothelial permeability by IL-1 and TNF- through TrkB. The single addition of BDNF into the culture increased the expression of VE-cadherin in HMVECs.ConclusionBDNF played an important role in inhibiting endothelial barrier dysfunction, which suggests that it may assist in enhancing periodontal tissue regeneration.