Infection with Mycobacterium pseudoshottsii in Cultured Yellowtail Seriola quinqueradiata in Owase Bay, Japan
Infection with Mycobacterium pseudoshottsii in Cultured Yellowtail Seriola quinqueradiata in Owase Bay, Japan
复制标题
DOI：
10.3147/jsfp.57.35
复制
发表时间：
2022-06-01
期刊：
影响因子：
0.6
通讯作者：
Kato, Goshi
中科院分区：
文献类型：
--
作者：
Matsumoto, Megumi;Machida, Yuichiro;Kato, Goshi
分枝杆菌病对日本黄尾鱼养殖业造成严重的经济损失。2020年10月至11月，日本三重县尾濑湾养殖的黄尾鱼发生分支杆菌病。本研究对病鱼进行了大体病理学和组织病理学观察。我们鉴定了从患病鱼中分离的分枝杆菌，并调查了它们对12种抗菌药物的药物敏感性。黄尾鱼的腹部肿胀，肾脏和脾脏中有许多结节。头肾和躯干肾呈现典型的肉芽肿，坏死核心被上皮样细胞和成纤维细胞包围。利用16S rRNA基因、hsp65和rpoB进行系统发育分析，将分离株鉴定为假肖氏分枝杆菌（Mycobacterium pseudoshottsii）。低浓度的丁胺卡那霉素、环丙沙星、卡那霉素和林可霉素对菌株生长有抑制作用。三个分离株中有两个对红霉素敏感，另一个对红霉素耐药。红霉素和林可霉素在日本被允许用于鲈形目的治疗，可能对治疗感染M.的黄尾鱼有用。拟肖氏菌然而，耐药菌株的出现，M。由于抗生素的使用，pseudoshottsii需要在黄尾水产养殖中引起注意。
Mycobacteriosis causes serious economic damages to yellowtail aquaculture in Japan. From October to November 2020, mycobacteriosis occurred in yellowtail cultured in Owase Bay, Mie Prefecture, Japan. In this study, we observed the gross pathology and histopathology of the diseased fish. We identified mycobacteria isolated from the diseased fish and investigated their drug sensitivities against 12 antimicrobial agents. Diseased yellowtail exhibited abdominal swelling and many nodules in their kidney and spleen. The head kidney and trunk kidney presented typical granulomas with a necrotic core surrounded by epithelioid cells and fibroblasts. The isolates were identified as Mycobacterium pseudoshottsii based on phylogenetic analysis using the 16S rRNA gene, hsp65 and rpoB. Low concentrations of amikacin, ciprofloxacin, kanamycin and lincomycin inhibited growth of the isolates. Two of three isolates showed sensitivity to erythromycin; the other isolate was resistant to it. Erythromycin and lincomycin that are permitted for therapeutic use in Perciformes in Japan may be;useful for treating yellowtail infected with M. pseudoshottsii. However, emergence of antibiotic-resistant strains of M. pseudoshottsii due to antibiotic use requires attention in yellowtail aquaculture.