On the substrate specificity of a damage-specific DNA binding protein from human cells.
On the substrate specificity of a damage-specific DNA binding protein from human cells.
复制标题
人类细胞损伤特异性 DNA 结合蛋白的底物特异性。
DOI：
10.1093/nar/8.5.1133
复制
发表时间：
1980
影响因子：
14.9
通讯作者：
Feldberg,RS
中科院分区：
文献类型：
--
作者：
Feldberg,RS
通过检查这种蛋白质的底物特异性，研究了由来自人细胞的损伤特异性DNA结合蛋白识别的病变的性质。在相对较低的UV能量密度下，蛋白质可识别的损伤被引入T7 DNA和Poly d（A-T）中。此外，蛋白质表现出结合亚硝酸和亚硫酸氢盐处理的DNA，但不与trioxsalen加上近紫外线交联的DNA，也不与未照射的含尿嘧啶的DNA。这些结果表明，这种蛋白质可以识别DNA中的微小螺旋扭曲，而不是任何一个单一的损伤。
The nature of the lesion recognized by a damage-specific DNA binding protein from human cells was investigated by examining the substrate specificity of this protein. Protein-recognizable damage was introduced into both T7 DNA and Poly d(A-T) at relatively low UV fluences. In addition, the protein demonstrated binding to both nitrous acid- and bisulfite-treated DNA, but not to DNA crosslinked with trioxsalen plus near-UV nor to non-irradiated uracil-containing DNA. These results suggest that this protein could be recognizing minor helix distortions in DNA rather than any one single lesion.