Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles
Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles
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DOI：
10.3109/17435390.2012.689880
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发表时间：
2013-08-01
期刊：
影响因子：
5
通讯作者：
Clark, Howard
中科院分区：
文献类型：
--
作者：
Kendall, Michaela;Ding, Ping;Clark, Howard
表面活性蛋白D(SP-D)主要在肺部表达，调节致炎和抗炎过程以应对毒性挑战，维持肺内环境的稳定。我们研究了NPs和SP-D之间的相互作用以及参与肺免疫的细胞随后的摄取。动态光散射(DLS)和扫描电子显微镜(SEM)测量了天然人SP-D(NhSP-D)和重组片段SP-D(rfhSP-D)的NP聚集性、颗粒大小和电荷。SP-D聚集的NPs，特别是在添加钙之后。免疫组织化学方法检测A549上皮细胞NPs与rfhSP-D的共定位。RfhSP-D促进NPs在体外与上皮A549细胞的共定位。比较了C57BL/6和SP-D基因敲除小鼠肺泡巨噬细胞(AM)和肺树突状细胞(LDC)对NP的摄取。AM和LDC显示与野生型小鼠相比，SP-D缺陷小鼠对NPs的摄取减少。这些数据证实了SP-D和NPs之间的相互作用，以及随后增强的NP摄取。
Surfactant protein D (SP-D) is primarily expressed in the lungs and modulates pro- and anti-inflammatory processes to toxic challenge, maintaining lung homeostasis. We investigated the interaction between NPs and SP-D and subsequent uptake by cells involved in lung immunity. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) measured NP aggregation, particle size and charge in native human SP-D (NhSP-D) and recombinant fragment SP-D (rfhSP-D). SP-D aggregated NPs, especially following the addition of calcium. Immunohistochemical analysis of A549 epithelial cells investigated the co-localization of NPs and rfhSP-D. rfhSP-D enhanced the co-localisation of NPs to epithelial A549 cells in vitro. NP uptake by alveolar macrophages (AMs) and lung dendritic cells (LDCs) from C57BL/6 and SP-D knock-out mice were compared. AMs and LDCs showed decreased uptake of NPs in SP-D deficient mice compared to wild-type mice. These data confirmed an interaction between SP-D and NPs, and subsequent enhanced NP uptake.