X-ray tomography generates 3-D reconstructions of the yeast, Saccharomyces cerevisiae, at 60-nm resolution
X-ray tomography generates 3-D reconstructions of the yeast, Saccharomyces cerevisiae, at 60-nm resolution
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DOI：
10.1091/mbc.e03-07-0522
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发表时间：
2004-03-01
影响因子：
3.3
通讯作者：
Le Gros, MA
中科院分区：
文献类型：
--
作者：
Larabell, CA;Le Gros, MA
我们研究了酵母，酿酒酵母，使用X射线断层扫描，并展示了这些细胞的内部结构组织在60纳米分辨率的独特观点。冷冻X射线断层扫描是一种新的成像技术，它可以产生整个细胞的三维（3-D）信息。在用于检查细胞的X射线的能量范围内，有机材料的吸收比水强大约一个数量级。这在完全水合的细胞中产生了可量化的天然对比度，并且消除了对化学固定剂或对比度增强试剂的需要以可视化细胞结构。因为可以使用免疫金标记方案在X射线显微镜中定位蛋白质（Meyer-Ilse等人，2001. J. Microsc. 201，395-403），断层扫描能够实现3-D分子定位。收集此处显示的每个单元格的数据所需的时间为
We examined the yeast, Saccharomyces cerevisiae, using X-ray tomography and demonstrate unique views of the internal structural organization of these cells at 60-nm resolution. Cryo X-ray tomography is a new imaging technique that generates three-dimensional (3-D) information of whole cells. In the energy range of X-rays used to examine cells, organic material absorbs approximately an order of magnitude more strongly than water. This produces a quantifiable natural contrast in fully hydrated cells and eliminates the need for chemical fixatives or contrast enhancement reagents to visualize cellular structures. Because proteins can be localized in the X-ray microscope using immunogold labeling protocols (Meyer-Ilse et al., 2001. J. Microsc. 201, 395-403), tomography enables 3-D molecular localization. The time required to collect the data for each cell shown here was