Multiple Gene Transformation of Rice Using the Biolistic Method
Multiple Gene Transformation of Rice Using the Biolistic Method
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发表时间：
1998
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通讯作者：
Tian Wen-zhong
中科院分区：
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作者：
Tian Wen-zhong
为建立水稻多基因转化体系，采用基因枪技术将GUS、hpt和RTBV CP基因分别置于不同质粒上，同时导入水稻细胞。在获得的25株转基因植株中，有3株（N12、K4-39和K1-1-66）的基因组中存在上述3个基因。两株可育，另一株不育。对一株三转植株（N12）的遗传分析表明，GUS和hpt基因紧密连锁在一条染色体上，而RTBV CP基因插入在另一条染色体上。
To establish a multiple gene transformation system for rice, experiments were carried out to deliver 3 genes (GUS, hpt and RTBV CP genes), located on different plasmids, simultaneously to rice cells using the biolistic method. In the obtained 25 transgenic plants, 3 (N12, K4-39 and K1-1-66) were demonstrated the presence of above 3 genes in their genomes. Two plants were fertile and another one was sterile. Genetic investigation of one tritransformed plant (N12) revealed that GUS and hpt genes are closely linked on one chromosome while RTBV CP gene is inserted in another chromosome.