Immunoexpression of aquaporin-1 in the efferent ducts of the rat and marmoset monkey during development, its modulation by estrogens, and its possible role in fluid resorption.
Immunoexpression of aquaporin-1 in the efferent ducts of the rat and marmoset monkey during development, its modulation by estrogens, and its possible role in fluid resorption.
复制标题
DOI：
10.1210/endo.139.9.6213
复制
发表时间：
1998-09
期刊：
影响因子：
4.8
通讯作者：
J. Fisher;K. J. Turner;H. M. Fraser;Philippa T. K. Saunders;Dennis Brown;Richard M. Sharpe
中科院分区：
文献类型：
--
作者：
J. Fisher;K. J. Turner;H. M. Fraser;Philippa T. K. Saunders;Dennis Brown;Richard M. Sharpe
最近的数据表明，雌激素在调节流出管的液体再吸收中发挥作用，尽管所涉及的生化机制尚不清楚。本研究采用免疫细胞化学定位水通道蛋白，水通道蛋白-1（AQP-1），从围产期到成年期的雄性大鼠和绒猴的输出管，然后调查其潜在的激素调节新生儿/青春期前后的生活，通过管理的GnRH拮抗剂（GnRHa）或己烯雌酚（DES）大鼠。AQP-1的免疫表达强烈的上皮细胞衬里的输出管在所有年龄段的研究，从胎儿晚期到青春期到成年期的顶端刷状缘。在绒猴，但不是大鼠，AQP-1也表达在睾丸网上皮。一旦传出导管上皮内的细胞类型已经分化，很明显，只有无纤毛细胞的大鼠本地化AQP-1。当促性腺激素分泌抑制大鼠的GnRHa的新生儿管理，AQP-1的免疫表达在年龄18和25天的强度几乎不变，但输出管的大小减少。相比之下，当DES给药大鼠（至第12天），AQP-1的免疫表达在第10天减少，在第18天几乎消失，在第25天显着减少，并在第35天在很小程度上，这些发现证实了Western印迹分析在第18天。DES诱导的AQP-1免疫表达的减少伴随着传出导管和网的明显扩张，与减少的液体吸收一致。DES处理大鼠的输出管上皮细胞呈立方形，而不是柱状的形状，在控制和显着降低的高度与控制相比，在所有年龄段，直到成年。这些研究结果表明，雌激素可能在调节胎儿/新生儿发育过程中的输出管的液体再吸收和/或在输出管和睾丸网的总体和功能发育中发挥作用。目前的数据还表明，水通道蛋白-1是参与调节的传出导管中的液体再吸收的元素之一。这些观察结果也表明了男性流出道系统在胎儿/新生儿发育中的流体流动的重要性。
Recent data suggest that estrogens play a role in regulating fluid resorption from the efferent ducts, though the biochemical mechanisms involved are unknown. The present study has used immunocytochemistry to localize a water channel protein, Aquaporin-1 (AQP-1), to the efferent ducts of male rats and marmoset monkeys from perinatal life through to adulthood and has then investigated its potential hormonal regulation in neonatal/peripubertal life, via administration of a GnRH antagonist (GnRHa) or diethylstilbestrol (DES) to rats. AQP-1 was immunoexpressed intensely in the apical brush border of the epithelium lining the efferent ducts at all ages studied, from late fetal life through puberty to adulthood. In the marmoset, but not the rat, AQP-1 was also expressed in the epithelium of the rete testis. Once the cell types within the efferent duct epithelium had differentiated, it was clear that only nonciliated cells of the rat localized AQP-1. When gonadotropin secretion was suppressed in rats by neonatal administration of GnRHa, immunoexpression of AQP-1 at age 18 and 25 days was virtually unchanged in intensity, though the efferent ducts were reduced in size. In contrast, when DES was administered neonatally to rats (up to day 12), immunoexpression of AQP-1 was reduced at day 10, virtually abolished at day 18, reduced markedly at day 25 and to a small extent at day 35; these findings were confirmed by Western blot analysis at day 18. The DES-induced decrease in immunoexpression of AQP-1 was accompanied by pronounced distension of the efferent ducts and rete, consistent with reduced fluid resorption. The epithelial cells of the efferent ducts in DES-treated rats were cuboidal rather than columnar in shape as in controls and were reduced significantly in height compared with controls at all ages through to adulthood. These findings suggest that estrogens may play a role in regulating fluid resorption from the efferent ducts during fetal/neonatal development and/or a role in the gross and functional development of the efferent ducts and rete testis. The present data also suggest that AQP-1 is one of the elements involved in the regulation of fluid resorption in the efferent ducts. The importance of fluid flow in fetal/neonatal development of the excurrent duct system of the male is also suggested by these observations.