Dynamic adhesion and separation of cells in vitro II. Interactions of cells with hydrophilic and hydrophobic surfaces
Dynamic adhesion and separation of cells in vitro II. Interactions of cells with hydrophilic and hydrophobic surfaces
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细胞体外动态粘附与分离II．
DOI：
10.1002/jcp.1040700104
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发表时间：
1967
影响因子：
5.6
通讯作者：
L. Blumenson
中科院分区：
文献类型：
--
作者：
Leonard Weiss;L. Blumenson
细胞通过未经处理的和硅化的玻璃珠传代的实验，以及培养细胞在玻璃和特氟龙表面的粘附和扩散的实验表明，在没有血清和低浓度血清存在的情况下，细胞的粘附和扩散对基质的润湿性很敏感。另一方面，在100%血清存在的情况下，黏附参数没有差异。由此得出的结论是，将细胞粘附性与表面润湿性以及由此推断的表面自由能相关联的论点在100%血清中是不成立的，这可能与体内情况很接近。
Experiments made on the passage of cells through untreated and siliconized glass beads, and on the adhesion and spread of cultured cells on glass and Teflon surfaces show that, in the absence of serum and in its presence in low concentrations, cell adhesion and spread is sensitive to substratum wettability. On the other hand, in the presence of 100% serum, no differences in adhesive parameters are detectable. It is concluded that arguments correlating cell adhesion to surface wettability, and, by inference, surface free energy, are unsubstantiated in 100% serum, which may well approximate to the in vivo situation.