CONTRIBUTION OF TRANSIENT BLOOD-FLOW TO TUMOR HYPOXIA IN MICE
CONTRIBUTION OF TRANSIENT BLOOD-FLOW TO TUMOR HYPOXIA IN MICE
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DOI：
10.3109/02841869509093982
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发表时间：
1995-01-01
期刊：
影响因子：
3.1
通讯作者：
LEPARD, NE
中科院分区：
文献类型：
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作者：
DURAND, RE;LEPARD, NE
在小鼠中生长的肿瘤通常表现出缺氧区域，据信这是由两种不同的过程引起的：由于消耗/扩散限制而导致的慢性缺氧，以及由于肿瘤血流短暂减少而导致的周期性缺氧。然而，每一种的相对贡献并不普遍。我们已经解决了这个问题，在移植的SCCVII鳞状细胞癌C3 H小鼠，使用荧光“错配”技术的定量扩展，再加上从照射肿瘤细胞分选。这些肿瘤中至少有一半的血管表现出短暂的灌注变化。此外，在SCCVII肿瘤中，15-20%的细胞中的大多数是足够缺氧的，对辐射有抵抗力，这似乎是由于周期性的，而不是连续的（扩散限制的）缺氧。由于可能需要不同的策略来抵消肿瘤中的循环缺氧，因此在计划人类癌症治疗时不应忽视瞬时血流变化的可能性。
Tumours grown in mice typically exhibit regions of hypoxia believed to result from two different processes: chronic oxygen deprivation due to consumption/diffusion limitations, and periodic deprivation resulting from transient reductions in tumour blood flow. The relative contribution of each is, however, not generally known. We have addressed this issue in transplanted SCCVII squamous cell carcinomas in C3H mice, using a quantitative extension of the fluorescence 'mismatch' technique coupled with cell sorting from irradiated tumours. At least half of the vessels in these tumours exhibit transient perfusion changes. Additionally, a majority of the 15-20% of cells that are sufficiently hypoxic to be resistant to radiation in the SCCVII tumours appear to result from cyclic, not continuous (diffusion-limited) hypoxia. Since different strategies may be necessary to counteract cyclic hypoxia in tumours, the possibility of transient blood flow changes should not be ignored when planning cancer therapy for humans.