NMR structural studies of membrane proteins.
NMR structural studies of membrane proteins.
复制标题
膜蛋白的核磁共振结构研究。
DOI：
10.1016/s0959-440x(98)80157-7
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发表时间：
1998
影响因子：
6.8
通讯作者：
Opella,SJ
中科院分区：
文献类型：
--
作者：
Marassi,FM;Opella,SJ
膜蛋白的三维结构对于理解其功能、相互作用和结构至关重要。它们对脂质的需求阻碍了传统方法的结构测定。与优化的样品，它是可能的应用溶液NMR方法胶束中的小膜蛋白;然而，脂质双层是膜蛋白的决定性环境，这需要固态NMR方法。新开发的固态核磁共振实验，使完全解决光谱从均匀同位素标记的磷脂脂质双层膜蛋白。由此产生的操作约束可以用于膜蛋白的结构的测定。
The three-dimensional structures of membrane proteins are essential for understanding their functions, interactions and architectures. Their requirement for lipids has hampered structure determination by conventional approaches. With optimized samples, it is possible to apply solution NMR methods to small membrane proteins in micelles; however, lipid bilayers are the definitive environment for membrane proteins and this requires solid-state NMR methods. Newly developed solid-state NMR experiments enable completely resolved spectra to be obtained from uniformly isotopically labeled membrane proteins in phospholipid lipid bilayers. The resulting operational constraints can be used for the determination of the structures of membrane proteins.