Phenotype-dependent response of cultured aortic smooth muscle to serum mitogens.
Phenotype-dependent response of cultured aortic smooth muscle to serum mitogens.
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DOI：
10.1083/jcb.89.2.379
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发表时间：
1981-05
期刊：
影响因子：
--
通讯作者：
Ross R
中科院分区：
文献类型：
--
作者：
Chamley-Campbell JH;Campbell GR;Ross R
来自成年猪和猴的主动脉中膜的平滑肌细胞通过接种从完整主动脉酶促解离的细胞而在原代培养物中生长。在前6天，这些细胞处于“收缩”表型。也就是说，它们响应于血管紧张素II而缓慢收缩，并且它们的细胞质充满了厚的和薄的肌丝。在这种状态下，它们不会将[3H]胸苷掺入DNA中，也不会对正常脂血症或高脂血症的全血血清（WBS）作出反应而增殖。在培养7天后，细胞经历表型到“合成”状态的自发调节，其中它们不能被刺激收缩，并且它们的细胞质充满了通常与分泌蛋白合成相关的细胞器。无法再显示含有粗肌球蛋白的细丝。当用正常脂血或高脂血WBS激发时，细胞将[3H]胸苷掺入DNA中并进行对数生长。有人建议，当平滑肌是收缩表型（如通常存在的大多数细胞在成年动物的主动脉媒体），它不分裂时，挑战血清有丝分裂原，但可以经历一个表型的变化，以合成状态，分裂可以被刺激。
Smooth muscle cells from the aortic media of adult pigs and monkeys have been grown in primary culture by plating cells enzymatically dissociated from the intact aorta. During the first 6 d these cells are in the "contractile" phenotype. That is, they contract slowly in response to angiotensin II and their cytoplasm is filled with both thick and thin myofilaments. In this state they do not incorporate [3H]thymidine into DNA or proliferate in response to normolipemic or hyperlipemic whole blood serum (WBS). After 7 d in culture the cells undergo a spontaneous modulation of phenotype to a "synthetic" state where they cannot be stimulated to contract and their cytoplasm is filled with organelles usually associated with synthesis of secretory protein. Thick myosin-containing filaments can no longer be demonstrated. When challenged with normolipemic or hyperlipemic WBS the cells incorporate [3H]thymidine into DNA and undergo logarithmic growth. It is suggested that when smooth muscle is the contractile phenotype (as normally exists for most cells in the aortic media of adult animals) it does not divide when challenged with serum mitogens but can undergo a change of phenotype to a synthetic state in which division can be stimulated.