Scission of RNA by the chemical nuclease of 1,10-phenanthroline-copper ion: preference for single-stranded loops.
Scission of RNA by the chemical nuclease of 1,10-phenanthroline-copper ion: preference for single-stranded loops.
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1,10-菲咯啉-铜离子化学核酸酶对 RNA 的断裂：优先选择单链环。
DOI：
10.1093/nar/17.13.5361
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发表时间：
1989
影响因子：
14.9
通讯作者：
Sigman,DS
中科院分区：
文献类型：
--
作者：
Murakawa,GJ;Chen,CH;Kuwabara,MD;Nierlich,DP;Sigman,DS
用lac mRNA片段和tRNAPheas底物研究了1，10-菲咯啉-铜（OP-Cu）的化学核酸酶活性对RNA的切割作用。由于断裂的化学机制涉及对核糖的氧化攻击，因此在包括二氢尿苷和Y碱基的所有核苷酸处观察到断裂。使用lac mRNA片段作为底物，OP-Cu与单链特异性试剂硫酸二甲酯和焦碳酸二乙酯的反应性相似，因此对单链环区域的特异性是显而易见的。在与tRNA的反应中观察到类似的偏好，尽管也观察到螺旋受体茎的断裂。
The scission of RNA by the chemical nuclease activity of 1,10-phenanthroline-copper (OP-Cu) has been studied using a lac mRNA fragment and tRNAPheas substrates. Since the chemical mechanism of scission involves oxidative attack on the ribose, scission is observed at all nucleotides including dihydrouridine and Y-bases. Specificity for single-stranded loop regions is apparent from the similarity of the reactivity of OP-Cu to the single-strand specific reagents dimethyl sulfate and diethyl pyrocarbonate using the fragment of lac mRNA as a substrate. Similar preference is observed in the reaction with tRNA although scission in the helical acceptor stem is also observed.