The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis.
The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis.
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DOI：
10.1111/j.1365-313x.2011.04785.x
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Yanbing Wang;Yingnan Hou;H. Gu;D. Kang;Zhang-liang Chen;Jingjing Liu;Li-jia Qu
中科院分区：
文献类型：
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作者：
Yanbing Wang;Yingnan Hou;H. Gu;D. Kang;Zhang-liang Chen;Jingjing Liu;Li-jia Qu
后期促进复合物/环体（APC/C）是一种E3泛素连接酶，参与调节细胞周期进程。它已在酵母和动物细胞中被广泛研究，但APC/C在植物细胞中的功能和调节在很大程度上是未知的。拟南芥APC/C包含至少11个亚基，其中只有少数已被详细研究。APC 4被认为是酵母和动物APC/C中的连接子。在这里，我们报告的拟南芥APC 4蛋白的功能特性。我们检测了三个携带apc 4等位基因的杂合植物品系。这些植物在生殖过程中表现出多效性发育缺陷，包括发育中胚囊的异常核行为和胚胎中的异常细胞分裂;这些表型不同于其他亚基突变体的报告。apc 4/+植株的一些胚珠和胚也积累了APC/C的已知底物cyclin B蛋白，表明APC/C的功能受损。APC 4在拟南芥幼苗的分生组织细胞、雌蕊中的胚珠和角果中的胚中均有表达，主要定位于细胞核。此外，生长素在apc 4/+植株的部分胚中的分布也发生了畸变。我们的研究结果表明，拟南芥APC 4在雌性配子发生和胚胎发生中起着至关重要的作用，可能是APC/C中的连接器，并且生长素分布的调节可能参与这些过程。
The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase that is involved in regulating cell-cycle progression. It has been widely studied in yeast and animal cells, but the function and regulation of the APC/C in plant cells are largely unknown. The Arabidopsis APC/C comprises at least 11 subunits, only a few of which have been studied in detail. APC4 is proposed to be a connector in the APC/C in yeast and animals. Here, we report the functional characterization of the Arabidopsis APC4 protein. We examined three heterozygous plant lines carrying apc4 alleles. These plants showed pleiotropic developmental defects in reproductive processes, including abnormal nuclear behavior in the developing embryo sac and aberrant cell division in embryos; these phenotypes differ from those reported for mutants of other subunits. Some ovules and embryos of apc4/+ plants also accumulated cyclin B protein, a known substrate of APC/C, suggesting a compromised function of APC/C. Arabidopsis APC4 was expressed in meristematic cells of seedlings, ovules in pistils and embryos in siliques, and was mainly localized in the nucleus. Additionally, the distribution of auxin was distorted in some embryos of apc4/+ plants. Our results indicate that Arabidopsis APC4 plays critical roles in female gametogenesis and embryogenesis, possibly as a connector in APC/C, and that regulation of auxin distribution may be involved in these processes.