The single-stranded DNA-binding protein of Deinococcus radiodurans.
The single-stranded DNA-binding protein of Deinococcus radiodurans.
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DOI：
10.1186/1471-2180-4-2
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发表时间：
2004-01-12
期刊：
影响因子：
4.2
通讯作者：
Cox MM
中科院分区：
文献类型：
--
作者：
Eggington JM;Haruta N;Wood EA;Cox MM
耐辐射异常球菌R1是已知最耐辐射的生物之一，能够修复异常大量的DNA损伤而不诱发突变。单链DNA结合蛋白（single-strandedDNA-bindingprotein，SSB）是所有生物体的必需蛋白质，参与DNA的复制、重组和修复。已发表的耐辐射异常球菌基因组序列包括一个推定的单链DNA结合蛋白基因（ssb; DR 0100），需要翻译移码合成一个完整的SSB蛋白。这个明显的三重基因在文献中激发了大量关于潜在的新移码或RNA编辑机制的猜测。紧接在ssb基因上游的是另一个基因（DR 0099），其给出了类似ssb的注释，但未被探索。耐辐射异常球菌菌株R1基因组的一个片段包含ssb基因已被重新测序，并记录了两个涉及遗漏鸟嘌呤核苷酸的错误。校正的序列将指定为DR 0099和DR 0100的开放阅读框并入一个连续的ssb开放阅读框（ORF）。校正的基因不需要翻译移码，并包含两个预测的寡核苷酸/寡糖结合（OB）倍。该蛋白已被纯化，其序列与嗜热栖热菌和水生栖热菌SSB蛋白密切相关。与栖热菌SSB蛋白一样，SSBDr作为同源二聚体发挥功能。耐辐射球菌SSB同二聚体以至少与大肠杆菌SSB同四聚体相同的效率刺激耐辐射球菌RecA蛋白和大肠杆菌RecA蛋白促进的DNA三链交换反应。正确的耐辐射异常球菌ssb基因是一个连续的开放阅读框，编码迄今为止鉴定的最大细菌SSB单体。耐辐射异常球菌SSB蛋白每个单体包含两个OB折叠，并作为同源二聚体发挥功能。耐辐射球菌SSB蛋白有效地刺激耐辐射球菌RecA以及大肠杆菌RecA蛋白促进的DNA链交换反应。对Deinococcus radiodurans SSB蛋白的鉴定和纯化不仅可以更好地理解SSB蛋白家族，而且在目前理解Deinococcus radiodurans非凡的DNA修复能力的努力中提供了一个重要但以前缺失的参与者。
Deinococcus radiodurans R1 is one of the most radiation-resistant organisms known and is able to repair an unusually large amount of DNA damage without induced mutation. Single-stranded DNA-binding (SSB) protein is an essential protein in all organisms and is involved in DNA replication, recombination and repair. The published genomic sequence from Deinococcus radiodurans includes a putative single-stranded DNA-binding protein gene (ssb; DR0100) requiring a translational frameshift for synthesis of a complete SSB protein. The apparently tripartite gene has inspired considerable speculation in the literature about potentially novel frameshifting or RNA editing mechanisms. Immediately upstream of the ssb gene is another gene (DR0099) given an ssb-like annotation, but left unexplored. A segment of the Deinococcus radiodurans strain R1 genome encompassing the ssb gene has been re-sequenced, and two errors involving omitted guanine nucleotides have been documented. The corrected sequence incorporates both of the open reading frames designated DR0099 and DR0100 into one contiguous ssb open reading frame (ORF). The corrected gene requires no translational frameshifts and contains two predicted oligonucleotide/oligosaccharide-binding (OB) folds. The protein has been purified and its sequence is closely related to the Thermus thermophilus and Thermus aquaticus SSB proteins. Like the Thermus SSB proteins, the SSBDr functions as a homodimer. The Deinococcus radiodurans SSB homodimer stimulates Deinococcus radiodurans RecA protein and Escherichia coli RecA protein-promoted DNA three-strand exchange reactions with at least the same efficiency as the Escherichia coli SSB homotetramer. The correct Deinococcus radiodurans ssb gene is a contiguous open reading frame that codes for the largest bacterial SSB monomer identified to date. The Deinococcus radiodurans SSB protein includes two OB folds per monomer and functions as a homodimer. The Deinococcus radiodurans SSB protein efficiently stimulates Deinococcus radiodurans RecA and also Escherichia coli RecA protein-promoted DNA strand exchange reactions. The identification and purification of Deinococcus radiodurans SSB protein not only allows for greater understanding of the SSB protein family but provides an essential yet previously missing player in the current efforts to understand the extraordinary DNA repair capacity of Deinococcus radiodurans.
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影响因子：
4.8
作者：
Haruta, N;Yu, XN;Cox, MM
通讯作者：
Cox, MM
DOI：
10.1073/pnas.94.13.6652
发表时间：
1997-06-24
影响因子：
11.1
作者：
Raghunathan, S;Ricard, CS;Waksman, G
通讯作者：
Waksman, G
DOI：
10.1073/pnas.172170199
发表时间：
2002-08-20
影响因子：
11.1
作者：
Lipton, MS;Pasa-Tolic, L;Smith, RD
通讯作者：
Smith, RD
影响因子：
2.7
作者：
Matsumoto, T;Morimoto, Y;Yasuoka, N
通讯作者：
Yasuoka, N
影响因子：
4.8
作者：
Lusetti, SL;Wood, EA;Cox, MM
通讯作者：
Cox, MM