PARASITOID VIRUS-LIKE PARTICLES DESTROY DROSOPHILA CELLULAR-IMMUNITY
PARASITOID VIRUS-LIKE PARTICLES DESTROY DROSOPHILA CELLULAR-IMMUNITY
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DOI：
10.1073/pnas.87.21.8388
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发表时间：
1990-11-01
影响因子：
11.1
通讯作者：
RIZKI, TM
中科院分区：
文献类型：
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作者：
RIZKI, RM;RIZKI, TM
寄生蜂必须避开寄主细胞防御系统的破坏作用，才能利用寄主血液作为其生存的适宜环境。为了保护它们的卵不被黑腹果蝇血细胞包裹，雌性异瘤Leptopilina注入一种能选择性破坏层状细胞的因子，层状细胞是果蝇血细胞的一种类型，参与识别和包裹大型异物。其他类型的宿主血细胞，包括吞噬浆细胞，保持功能。本报告表明，层状细胞的破坏因子是一种病毒样颗粒（VLP），储存在与雌蜂生殖系统相关的副腺储存库中。我们证明了VLPs在体外进入果蝇血细胞。VLPs游离于层状细胞的细胞质中，但局限于浆细胞的吞噬囊泡中。由于层状细胞易受VLP感染，而浆细胞不易，因此我们认为VLP进入细胞质的方式及其在细胞质中的分布决定了被感染宿主血细胞的命运。
Parasitoid wasps must avoid the destructive effects of the host''s cellular defense system in order to exploit the host hemocoel as a suitable environment for their survival. To protect their eggs from encapsulation by Drosophila melanogaster blood cells, Leptopilina heterotoma females inject a factor that selectively destroys lamellocytes, the type of Drosophila blood cell involved in recognition and encapsulation of large foreign objects. Other types of host blood cells, including the phagocytic plasmatocytes, remain functional. This report demonstrates that the destructive factor for lamellocytes is a virus-like particle (VLP) stored in the reservoir of an accessory gland associated with the female wasp reproductive system. We show that VLPs enter Drosophila blood cells in vitro. VLPs are found free in the cytoplasm of lamellocytes but are confined to phagocytic vesicles of plasmatocytes. As lamellocytes are susceptible to the VLP infection and plasmatocytes are not, we conclude that the mode of VLP entry and its disposition in the cytoplasm determine the fate of the infected host blood cell.