Internalization of E. coli ST mediated by guanylyl cyclase C in T84 human colon carcinoma cells.
Internalization of E. coli ST mediated by guanylyl cyclase C in T84 human colon carcinoma cells.
复制标题
T84 人结肠癌细胞中鸟苷酸环化酶 C 介导的大肠杆菌 ST 内化。
DOI：
10.1016/0304-4165(95)00068-m
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Waldman,SA
中科院分区：
文献类型：
--
作者：
Urbanski,R;Carrithers,SL;Waldman,SA
Internalization of Escherichia coli heat-stable enterotoxin (ST) mediated by guanylyl cyclase C was examined in T84 human colon carcinoma cells. Surface-associated, receptor-bound ST was quantitatively separated from intracellular ligand employing acidic guanidine-HCl. ST was internalized in a time-, temperature-, and ligand concentration-dependent fashion only by cells specifically expressing guanylyl cyclase C. Only receptors which bound reversibly to ST appeared to mediate endocytosis. The rate of internalization of ST empirically determined in these studies was 0.23 min−1. The density of surface receptors for ST was similar at 4°C and 37°C, suggesting that these receptors recycle back to the cell surface following internalization of ligand. Similarly, internalized ST was rapidly cleared from the intracellular compartment following endocytosis. These studies demonstrate that ST undergoes ligand-dependent receptor-mediated endocytosis in human colon carcinoma cells.
