Comparative chronic toxicities of PFOS and its novel alternatives on the immune system associated with intestinal microbiota dysbiosis in adult zebrafish
Comparative chronic toxicities of PFOS and its novel alternatives on the immune system associated with intestinal microbiota dysbiosis in adult zebrafish
复制标题
全氟辛烷磺酸及其新型替代品对成年斑马鱼肠道菌群失调相关免疫系统的慢性毒性比较
DOI：
10.1016/j.jhazmat.2021.127950
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发表时间：
2021-12-08
影响因子：
13.6
通讯作者：
Tu, Wenqing
中科院分区：
文献类型：
--
作者：
Huang, Jing;Wang, Qiyu;Tu, Wenqing
6：2氯化多氟醚磺酸盐（F-53 B）和对全氟壬烯氧基苯磺酸钠（OBS）在中国市场上被广泛用作全氟辛烷磺酸（PFOS）的替代品。在这里，成年斑马鱼长期暴露于1 μ M的全氟辛烷磺酸，F-53 B，和OBS的21天，研究这三个全氟烷基和多氟烷基物质（PFAS）的比较免疫毒性。全氟辛烷磺酸在肝脏中引起的氧化应激比F-53 B和OBS更严重，这三种全氟辛烷磺酸通过抑制促炎细胞因子的表达产生了类似的抗炎作用。肠道微生物群分析表明，邻单胞菌属、气单胞菌属、鲸杆菌属、希瓦氏菌属和弧菌属的相对丰度在三个PFAS处理组中以相同的趋势变化。此外，PFAS还可增加肠道中hepcidin、muc、免疫相关基因mpo和saa的表达，降低紧密连接相关基因occ的表达，并使肠道绒毛高度降低，表明肠道功能受到破坏。特别是，肠道菌群变化与肝脏和肠道指标之间的显著相关性也表明了免疫系统与肠道菌群之间的相互作用。综上所述，我们的研究结果表明，暴露于全氟辛烷磺酸及其替代品F-53 B和OBS可诱导成年斑马鱼与肠道微生物群生态失调相关的肝脏免疫毒性。
6:2 Chlorinated polyfluorinated ether sulfonate (F-53B) and sodium p-perfluorous nonenoxybenzene sulfonate (OBS) are widely used as perfluorooctane sulfonate (PFOS) alternatives in the Chinese market. Here, adult zebrafish were chronically exposed to 1 mu M PFOS, F-53B, and OBS for 21 days to investigate the comparative immunotoxicity of these three per-and polyfluoroalkyl substances (PFAS). PFOS induced more severe oxidative stress in the liver than F-53B and OBS, and these three PFAS induced similar anti-inflammatory effects by repressing the expression of pro-inflammatory cytokines. The intestinal microbiota analysis showed that the relative abundance of Plesiomonas, Aeromonas, Cetobacterium, Shewanella, and Vibrio changed with the same trend in the three PFAS treatment groups. Furthermore, the PFAS increased the expression of hepcidin, muc, the immune-related genes mpo and saa, and decreased the expression of the tight junction-related gene occ in the intestine; moreover, villus height of the intestine was reduced after PFAS exposure, which indicated the functional disruption of the intestine. In particular, the significant correlation between the changed intestinal microbiota and liver and intestinal indicators also suggested the interaction between the immune system and intestinal microbiota. Taken together, our results indicate that exposure to PFOS and its alternatives F-53B and OBS can induce hepatic immunotoxicity associated with intestinal microbiota dysbiosis in adult zebrafish.