Quorum quenching acylase impacts the viability and morphological change of Agrobacterium tumefaciens cells
Quorum quenching acylase impacts the viability and morphological change of Agrobacterium tumefaciens cells
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DOI：
10.1016/j.jbiosc.2020.02.005
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发表时间：
2020-07-01
影响因子：
2.8
通讯作者：
Terada, Akihiko
中科院分区：
文献类型：
--
作者：
Bao, Qian;Hosoe, Ayaka;Terada, Akihiko
酰化酶是一种群体淬灭酶，它降解N-酰基高丝氨酸内酯（AHL），这是群体感应（QS）机制中的关键信号分子。酰化酶I切割AHL化学结构中的酰基链，从而通过抑制细菌细胞通讯和胞外聚合物（EPS）的分泌来发挥抗生物膜作用。然而，酰化酶对细菌细胞的物理和生理影响仍有待系统阐明。因此，本研究调查了活性和非活性酰化酶添加对根癌农杆菌的生长、活力和细胞形态的影响。为了进行比较，将活性和非活性溶菌酶作为阳性对照。结果表明，活性酰化酶抑制A.在0.1 - 1000 μ g/mL浓度范围内，对根癌农杆菌细胞生长有抑制作用，活性溶菌酶也有抑制作用。通过活/死染色的荧光检测支持A.当浓度高于0.1 μ g mL（-1）时，酰化酶和溶菌酶的活性均下降，尽管溶菌酶造成了更高程度的细胞损伤。此外，原子力显微镜揭示了A。tumefaciens细胞的酶活性。总之，结果表明酰化酶不仅阻断了基于AHL的QS机制，而且损害了细胞活力并改变了A.根癌细胞，如通过加入水解酶所观察到的。(C)2020年，生物技术学会，日本。All rights reserved.
Acylase is known as a quorum quenching enzyme that degrades N-acyl-homoserine lactones (AHLs), a key signaling molecule in a quorum sensing (QS) mechanism. Acylase I cleaves the acyl-chain in the chemical structures of AHLs, thereby exerting an anti-biofilm effect by the inhibition of bacterial cellecell communication and resultant secretion of extracellular polymeric substances (EPS). However, the physical and physiological impacts of acylase on bacterial cells remain to be systematically elucidated. This study, therefore, investigated the effect of active and inactive acylase addition on the growth, viability, and cell morphologies of Agrobacterium tumefaciens. For comparison, active and inactive lysozymes were taken as positive controls. The results showed that active acylase inhibited A. tumefaciens cell growth at concentrations ranging from 0.1 to 1000 mu g mL(-1), and so did active lysozyme. Fluorescent detection by Live/Dead staining underpinned that cell viability of A. tumefaciens decreased at concentrations higher than 0.1 mu g mL(-1) for both acylase and lysozyme, although lysozyme inflicted higher degree of cellular damage. Moreover, atomic force microscopy unraveled a noticeable distortion of A. tumefaciens cells by both acylase and lysozyme. Together, the results showed that acylase not only blocked AHLs-based QS mechanisms but also compromised cell viability and altered surface morphology of A. tumefaciens cells, as observed by the addition of hydrolase. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.