Age-related loss of rooting capability in Arabidopsis thaliana and its reversal by peptides containing the Arg-Gly-Asp (RGD) motif
Age-related loss of rooting capability in Arabidopsis thaliana and its reversal by peptides containing the Arg-Gly-Asp (RGD) motif
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DOI：
10.1034/j.1399-3054.2002.1140414.x
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发表时间：
2002-04-01
影响因子：
6.4
通讯作者：
Sabater, B
中科院分区：
文献类型：
--
作者：
Díaz-Sala, C;Garrido, G;Sabater, B
本文描述了一个研究拟南芥不定根形成随年龄变化的实验系统。（Heynh）、生态型兰茨贝格直立（Ler）。该系统是基于不同的生根能力的下胚轴从去根的幼年（12天）和成年（26天）的植物。从去根的幼年植物的下胚轴生根容易在一周内的文化，和生根过程是不依赖于外源生长素。相比之下，来自去根的成年植物的下胚轴生根较差，并且仅在较长的时间段之后。外源生长素对成年植株去根后的下胚轴生根没有影响。生根能力，虽然与过渡到开花，并不依赖于这种过渡。根诱导下降以类似的方式时，过渡到开花延迟，无论是遗传与fve突变体或生理短日。结果表明，脱根植株下胚轴的生根依赖于含有RGD基序的多肽的作用。当下胚轴用RGD肽瞬时处理时，生根的百分比和根的数量都大大增加。RGD肽的作用是一个必要的，但不是充分的条件，从去根的成年植物下胚轴生根。
We describe here an experimental system to study the age-related decline of adventitious root formation in Arabidopsis thaliana L. (Heynh), ecotype Landsberg erecta (Ler). The system is based on the different rooting capacity of hypocotyls from de-rooted juvenile (12-day-old) and adult (26-day-old) plants. Hypocotyls from de-rooted juvenile plants rooted readily within a week of culture, and the rooting process was not dependent on exogenous auxin. In contrast, hypocotyls from de-rooted adult plants rooted poorly and only after longer periods of time. Exogenously applied auxin had no effect on rooting of hypocotyls from de-rooted adult plants. Rooting capacity, although correlated with the transition to flowering, did not depend on this transition. Root induction declined in a similar manner when the transition to flowering was delayed, either genetically with the fve mutant or physiologically with short days. The results showed that rooting of hypocotyls from de-rooted adult plants depended on the effect of peptides containing the RGD motif. Both the percentage of rooting and the number of roots were largely increased when the hypocotyls were treated transiently with the RGD peptide. The effect of the RGD peptide was a necessary, but not sufficient, condition for rooting of hypocotyls from de-rooted adult plants.