Fur is involved in manganese-dependent regulation of mntA (sitA) expression in Sinorhizobium meliloti
Fur is involved in manganese-dependent regulation of mntA (sitA) expression in Sinorhizobium meliloti
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DOI：
10.1128/aem.70.7.4349-4355.2004
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发表时间：
2004-07-01
影响因子：
4.4
通讯作者：
Fabiano, E
中科院分区：
文献类型：
--
作者：
Platero, R;Peixoto, L;Fabiano, E
Fur是一种转录调节因子，参与许多细菌中铁依赖性基因表达的控制。在这项工作中，我们分析了Sinorhizobium meliloti的一个皮毛突变体的表型，Sinorhizobium meliloti是一种与宿主植物相关的固定N-2的α -变形菌。我们证明了一些涉及高亲和力铁转运、铁载体产生和铁调控外膜蛋白表达的功能以不依赖皮毛的方式响应铁。然而，MntABCD锰转运系统的锰依赖表达在突变株中丢失，这可以通过mntA::gfp融合报告基因的组成性表达来识别。因此，Fur直接或间接调节了锰依赖的功能。这些数据表明细菌皮毛蛋白在介导锰依赖的基因表达调控中具有新功能。
Fur is a transcriptional regulator involved in iron-dependent control of gene expression in many bacteria. In this work we analyzed the phenotype of a fur mutant in Sinorhizobium meliloti, an alpha-proteobacterium that fixes N-2 in association with host plants. We demonstrated that some functions involved in high-affinity iron transport, siderophore production, and iron-regulated outer membrane protein expression respond to iron in a Fur-independent manner. However, manganese-dependent expression of the MntABCD manganese transport system was lost in a fur strain as discerned by constitutive expression of a mntA::gfp fusion reporter gene in the mutant. Thus, Fur directly or indirectly regulates a manganese-dependent function. The data indicate a novel function for a bacterial Fur protein in mediating manganese-dependent regulation of gene expression.