Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion
Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion
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利用 Cas9-胞苷脱氨酶融合对水稻、小麦和玉米进行精确碱基编辑
DOI：
10.1038/nbt.3811
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发表时间：
2017-05-01
影响因子：
46.9
通讯作者：
Gao, Caixia
中科院分区：
文献类型：
--
作者：
Zong, Yuan;Wang, Yanpeng;Gao, Caixia
在不需要外来DNA供体或双链DNA切割的情况下，在植物中进行有针对性的碱基编辑将加快基因组修改和在多种作物中的育种。我们使用CRISPR-Cas9尼克酶-胞苷脱氨酶融合，在原生质体和再生水稻、小麦和玉米植株中实现了胞嘧啶到胸腺嘧啶的定向转化，转化率高达43.48%。
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.