Laser-assisted microdissection of the zona pellucida facilitates polar body biopsy
Laser-assisted microdissection of the zona pellucida facilitates polar body biopsy
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DOI：
10.1016/s0015-0282(97)00538-4
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发表时间：
1998-03-01
影响因子：
6.7
通讯作者：
van der Ven, H
中科院分区：
文献类型：
--
作者：
Montag, M;van der Ven, K;van der Ven, H
目的：目的：探讨激光显微切割透明细胞（ZP）后能否进行极体活检。对受精卵进行ZP的激光显微切割和极体活检（第1组），单独激光显微切割（第2组），或不处理（第3组）。设置：基于大学的IVF程序。患者：动物研究。干预：使用1.48 μ m非接触式二极管激光在小鼠受精卵的ZP上钻孔。一个微针插入和极体被aspiration.Main结果Measure（S）：极体活检后的ZP的激光显微切割的疗效进行了evaluated. Results（S）：激光二极管光束允许精确钻孔的14- 18-μ m孔的ZP。极体可以被吸出而不损伤受精卵，并且在活检过程中不解体。受精卵发育成囊胚，并经历与对照受精卵相同的孵化。较低的孵化率，观察未处理的zigotes. Conclusions（S）：激光显微切割的ZP与非接触式激光系统有利于随后的极体活检。使用钝头微量移液器可大大降低受精卵或极体受损的风险。这一程序使极体活检更准确和有效的植入前遗传学诊断。(C)1998年，美国生殖医学会。
Objective: To investigate whether polar body biopsy can be performed after laser microdissection of the zona pellucida (ZP).Design: Mouse zygotes were allocated randomly to three groups. The zygotes were subjected to laser microdissection of the ZP and polar body biopsy (group 1), laser microdissection alone (group 2), or no treatment (group 3).Setting: University-based IVF program.Patient(s): Animal study.Intervention(s): A hole was drilled in the ZP of mouse zygotes using a 1.48-mu m noncontact diode laser. A microneedle was inserted and the polar body was aspirated.Main Outcome Measure(s): The efficacy of polar body biopsy after laser microdissection of the ZP was evaluated.Result(s): The laser diode beam allowed for precise drilling of a 14- to 18-mu m hole in the ZP. Polar bodies could be aspirated without damaging the zygote and did not disintegrate during the biopsy. Zygotes developed to blastocysts and underwent the same hatching as control zygotes. Lower hatching rates were observed in untreated zygotes.Conclusion(s): Laser microdissection of the ZP with a noncontact laser system facilitates subsequent polar body biopsy. The use of blunt-ended micropipettes greatly reduces the risk of damage to the zygote or the polar body. This procedure makes polar body biopsy more accurate and effective for preimplantation genetic diagnosis. (C) 1998 by American Society for Reproductive Medicine.