Characterization of DDAB/Cholesterol Vesicles and Its Comparison with Lipid/Cholesterol Vesicles
Characterization of DDAB/Cholesterol Vesicles and Its Comparison with Lipid/Cholesterol Vesicles
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DDAB/胆固醇囊泡的表征及其与脂质/胆固醇囊泡的比较
DOI：
10.1166/jnn.2018.14252
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发表时间：
2018
影响因子：
--
通讯作者：
Umakoshi Hiroshi
中科院分区：
文献类型：
--
作者：
Iwasaki Fumihiko;Suga Keishi;Okamoto Yukihiro;Umakoshi Hiroshi
用胆固醇对合成的表面活性剂DDAB（dilauryldimethylammoniumbromide）制备的囊泡进行修饰，并通过荧光探针Laurdan（6-lauroyl-2-dimethylaminonaphthalene）和DPH（1，6-diphenyl-1，3，5-hexatriene）的分析表征囊泡的膜表面性质。动态光散射分析表明，DDAB与胆固醇的自组装形成了平均直径为127 nm的稳定囊泡结构。DDAB囊泡具有高极性和高流动性，胆固醇对DDAB囊泡的修饰导致囊泡膜上形成“非均相”。DDAB：胆固醇= 70：30的囊泡显示出代表极性环境但较低流动性的独特特征。胆固醇修饰的人工表面活性剂囊泡有望为水介质中的化学过程提供一个新的平台。
Vesicles prepared by synthetic surfactant, DDAB (dilauryldimethylammonium bromide), were modified with cholesterol and their membrane surface properties of the vesicle were characterized through the analyses of fluorescent probes, such as Laurdan (6-lauroyl-2-dimethylaminonaphthalene) and DPH (1,6-diphenyl-1,3,5-hexatriene). The self-assembly of DDAB with cholesterol showed stable vesicle structure with a mean diameter of 127 nm through the dynamic light scattering analysis. While the DDAB vesicle showed high polarity and high fluidity, the modification of the DDAB vesicle with cholesterol lead to the formation of “heterogeneous phase” on the vesicle membrane. DDAB:cholesterol = 70:30 vesicle showed unique characteristics that represents polar environment but lower fluidity. A novel platform for the chemical process in aqueous media can be expected by using the artificial surfactant vesicles modified with cholesterol.