MRY, A TRANS-ACTING POSITIVE REGULATOR OF THE M-PROTEIN GENE OF STREPTOCOCCUS-PYOGENES WITH SIMILARITY TO THE RECEPTOR PROTEINS OF 2-COMPONENT REGULATORY SYSTEMS
MRY, A TRANS-ACTING POSITIVE REGULATOR OF THE M-PROTEIN GENE OF STREPTOCOCCUS-PYOGENES WITH SIMILARITY TO THE RECEPTOR PROTEINS OF 2-COMPONENT REGULATORY SYSTEMS
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DOI：
10.1128/jb.173.8.2617-2624.1991
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发表时间：
1991-04-01
影响因子：
3.2
通讯作者：
SCOTT, JR
中科院分区：
文献类型：
--
作者：
PEREZCASAL, J;CAPARON, MG;SCOTT, JR
在化脓性链球菌M6菌株D471中，接合转座子Tn916插入emm6（M蛋白的结构基因）启动子上游2kb的区域，使得该菌株变为M阴性（M. G. 卡帕隆和J. R. 斯科特，《美国国家科学院院刊》84:8677 - 8681，1987年）。在本研究中，我们表明这种插入突变mry - 1位于一个编码我们称为Mry的蛋白质的开放阅读框上游244bp处。该蛋白质在体外转录和翻译后在凝胶上可见。我们开发了一种用于化脓性链球菌互补分析的技术，并利用它表明野生型mry基因对两个突变等位基因呈显性。这种显性表明Mry作为emm6基因的正调控因子反式作用。mry的翻译DNA序列有两个区域与双组分调控系统的受体蛋白共有的基序相似。此外，Mry的N末端有两个类似螺旋 - 转角 - 螺旋基序的区域。Mry似乎不是A群链球菌中毒力决定因子的全局调控因子，因为mry - 1突变对透明质酸荚膜或链激酶的产生没有影响。
In the Streptococcus pyogenes M6 strain D471, an insertion of the conjugative transposon Tn916 into a region 2 kb upstream of the promoter of emm6 (the structural gene for the M protein) rendered the strain M negative (M. G. Caparon and J. R. Scott, Proc. Natl. Acad. Sci. USA 84:8677-8681, 1987). In the present work, we show that this insertion mutation, mry-1, is 244 bp upstream of an open reading frame encoding a protein we call Mry. This protein is visible on a gel after transcription and translation in vitro. We have developed a technique for complementation analysis in S. pyogenes and have used it to show that the wild-type mry gene is dominant to two mutant alleles. This dominance indicates that Mry acts in trans as a positive regulator of the emm6 gene. The translated DNA sequence of mry has two regions of similarity to the motif common to the receptor protein of two-component regulatory systems. In addition, the N terminus of Mry has two regions resembling a helix-turn-helix motif. Mry does not appear to be a global regulator of virulence determinants in the group A streptococcus because there is no effect of the mry-1 mutation on production of the hyaluronic acid capsule or streptokinase.