In situ deletions reveal regulatory components for expression of an intracellular immune receptor gene and its co‐expressed genes in Arabidopsis
In situ deletions reveal regulatory components for expression of an intracellular immune receptor gene and its co‐expressed genes in Arabidopsis
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原位缺失揭示了拟南芥细胞内免疫受体基因及其共表达基因表达的调控成分
DOI：
10.1111/pce.14293
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Hua, Jian
中科院分区：
文献类型：
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作者：
Yu, Huiyun;Yang, Leiyun;Li, Zhan;Sun, Feng;Li, Bo;Guo, Shengsong;Wang, Yong‐Fei;Zhou, Tong;Hua, Jian
细胞内免疫受体核苷酸结合富亮氨酸重复序列（NLR）在转录和转录后高度调节，以平衡植物防御和生长。NLR基因通常存在于基因簇中，并且通常在各种条件下共表达。尽管对NLR蛋白的调控进行了深入的研究，但NLR基因诱导、抑制或共表达的顺式作用元件在很大程度上是未知的，因为它们的表达调控的顺式区域比通常的大。在这里，我们使用CRISPR/Cas9基因组编辑技术在RPP 5基因簇中的NLR基因SNC 1的内源位置产生一系列原位缺失。这些在野生型和SNC 1组成型表达自身免疫性mucobon 1中的缺失揭示了SNC 1表达的阳性和阴性顺式作用元件。两个转录因子可以结合到这些元件被发现有SNC 1的表达的影响。此外，发现同一簇中两个基因与SNC 1的共表达主要依赖于SNC 1的功能。因此，SNC 1的表达受到复杂的局部调控，涉及多个顺式元件，SNC 1本身是同一基因簇中其他NLR基因表达的关键调控因子。
Intracellular immune receptor nucleotide‐binding leucine‐rich repeats (NLRs) are highly regulated transcriptionally and post‐transcriptionally for balanced plant defence and growth. NLR genes often exist in gene clusters and are usually co‐expressed under various conditions. Despite of intensive studies of regulation of NLR proteins, cis‐acting elements for NLR gene induction, repression or co‐expression are largely unknown due to a larger than usual cis‐region for their expression regulation. Here we used the CRISPR/Cas9 genome editing technology to generate a series of in situ deletions at the endogenous location of a NLR geneSNC1residing in theRPP5gene cluster. These deletions that made in the wild type and theSNC1constitutive expressing autoimmune mutantbon1revealed both positive and negative cis‐acting elements forSNC1expression. Two transcription factors that could bind to these elements were found to have an impact on the expression ofSNC1. In addition, co‐expression of two genes withSNC1in the same cluster is found to be mostly dependent on theSNC1function. Therefore,SNC1expression is under complex local regulation involving multiple cis elements andSNC1itself is a critical regulator of gene expression of other NLR genes in the same gene cluster.