What is the best wavelength for the measurement of hemolysis index?
What is the best wavelength for the measurement of hemolysis index?
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DOI：
10.1016/j.cca.2020.06.046
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发表时间：
2020-11-01
影响因子：
5
通讯作者：
Yamasaki, Takahiro
中科院分区：
文献类型：
--
作者：
Ishiguro, Akiyo;Nishioka, Mitsuaki;Yamasaki, Takahiro
背景：溶血是处理血清标本时常见的问题。溶血指数（HI）为自动分析仪提供溶血预警。但是，HI并没有标准化，各实验室都采用原有的方法。特别是，用于HI测量的波长在每个实验室是不同的。因此，我们研究了HI在不同波长下的预警能力。方法：选取4种波长类型，测量并计算每种波长类型的HI值（410 nm/HI-1、451 nm/HI-2、545 nm/HI-3、571 nm/HI-4）。为了比较4种HI类型，我们采用人工溶血标本（AHSs）研究了3种干扰成分的影响。我们还研究了HI与血红蛋白浓度（Hb）的关系以及HI与AHSs 31项生化试验值的关系。结果：在干扰评价中，只有HI-4对3种干扰成分无影响。Hb与HI-4的相关性非常强（r(S) = 0.9987）。1单位的HI-4增加对应于14.8 mg/dL的Hb增加。结论：HI的最佳波长在571 nm附近。
Background: Hemolysis is a common problem in the handling of serum specimens. The hemolysis index (HI) provides a warning of hemolysis in auto-analyzers. However, HI has not been standardized, and each laboratory's original method is applied. Especially, the wavelength used for HI measurement is different in each laboratory. Thus, we investigated the warning ability of HI at various wavelengths.Methods: We selected 4 wavelength types, and each HI was measured and calculated (410 nm/HI-1, 451 nm/HI-2, 545 nm/HI-3, and 571 nm/HI-4). To compare the 4 HI types, we investigated the influence of 3 interference components using artificially hemolyzed specimens (AHSs). We also investigated both the relationship between HI and hemoglobin concentration (Hb) and that between HI and 31 biochemical test values in AHSs.Results: In the interference assessment, only HI-4 showed no influence on the 3 interference components. The correlation between Hb and HI-4 was very strong (r(S) = 0.9987). A 1-unit increase in HI-4 corresponded to a 14.8-mg/dL increase in Hb.Conclusion: We found the best wavelength for HI to be at or near 571 nm.