ELAV, a Drosophila neuron-specific protein, mediates the generation of an alternatively spliced neural protein isoform
ELAV, a Drosophila neuron-specific protein, mediates the generation of an alternatively spliced neural protein isoform
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ELAV是果蝇神经元特异性蛋白，可介导产生一种替代剪接的神经蛋白异构体
DOI：
10.1016/s0960-9822(02)70787-2
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发表时间：
1996-12-01
期刊：
影响因子：
9.2
通讯作者：
White, K
中科院分区：
文献类型：
--
作者：
Koushika, SP;Lisbin, MJ;White, K
背景资料：组织特异性前体mRNA选择性剪接是基因调控和产生不同蛋白质亚型的一种广泛使用的机制，但对介导这一过程的因素和机制知之甚少。组织特异性RNA结合蛋白可以介导前体mRNA的选择性剪接，在果蝇中，由胚胎致死异常视觉系统（embryonic lethal abnormal visual system，elav）基因编码的RNA结合蛋白是一个候选者。ELAV蛋白只在神经元中表达，并且对于神经系统的形成和维持是重要的。结果：在这项研究中，光感受器神经元基因耗尽ELAV，和elav空中枢神经系统神经元，进行了免疫细胞化学分析神经蛋白的表达。在这两种情况下，ELAV的缺乏对应于神经胶质细胞的神经特异性亚型的免疫组织化学信号的减少，这是由选择性剪接产生的。此外，当ELAV异位表达的细胞，通常只表达非神经亚型的神经胶质细胞，我们观察到的神经亚型的Neuroglian.Conclusions：果蝇ELAV促进神经元特异性亚型的神经胶质细胞的产生通过调节前体mRNA剪接。本文报道的研究结果表明，ELAV是必要的，ELAV在椎间盘细胞中的异位表达足以介导神经元特异性选择性剪接。
Background: Tissue-specific alternative pre-mRNA splicing is a widely used mechanism for gene regulation and the generation of different protein isoforms, but relatively little is known about the factors and mechanisms that mediate this process. Tissue-specific RNA-binding proteins could mediate alternative pre-mRNA splicing, In Drosophila melanogaster, the RNA-binding protein encoded by the elav (embryonic lethal abnormal visual system) gene is a candidate for such a role. The ELAV protein is expressed exclusively in neurons, and is important for the formation and maintenance of the nervous system.Results: In this study, photoreceptor neurons genetically depleted of ELAV, and elav-null central nervous system neurons, were analyzed immunocytochemically for the expression of neural proteins. In both situations, the lack of ELAV corresponded with a decrease in the immunohistochemical signal of the neural-specific isoform of Neuroglian, which is generated by alternative splicing. Furthermore, when ELAV was expressed ectopically in cells that normally express only the non-neural isoform of Neuroglian, we observed the generation of the neural isoform of Neuroglian.Conclusions: Drosophila ELAV promotes the generation of the neuron-specific isoform of Neuroglian by the regulation of pre-mRNA splicing. The findings reported in this paper demonstrate that ELAV is necessary, and the ectopic expression of ELAV in imaginal disc cells is sufficient, to mediate neuron-specific alternative splicing.