Uniqueness of Stationary Equilibrium Payoffs in the Baron-Ferejohn Model
Uniqueness of Stationary Equilibrium Payoffs in the Baron-Ferejohn Model
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Baron-Ferejohn 模型中稳态均衡收益的独特性
DOI：
10.1006/jeth.2001.2820
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Hülya Eraslan
中科院分区：
文献类型：
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作者：
Hülya Eraslan
使用由2-烷酰氨基-4-硝基苯基磷酰胆碱氢氧化物组成的人工底物，通过将底物与提取物一起孵育来测定人体细胞或组织提取物中的鞘磷脂酶活性，由此鞘磷脂酶催化底物水解成相应的2-烷酰氨基-4-硝基苯酚和磷胆碱，其量与提取物中的鞘磷脂酶活性成正比。由此产生的2-烷基氨基-4-硝基苯酚的碱化作用将其转化为碱性盐，从而形成明亮的黄色，其强度与底物的水解量成正比。例如，通过简单的比色计或光度计测量黄色的强度，可以准确地指示提取物中的鞘磷脂酶活性。
An artificial substrate consisting of a 2-alkanoylamino-4-nitrophenyl phosphorylcholine hydroxide is used for determining the sphingomyelinase activity in an extract of human cells or tissues by incubating the substrate together with the extract, whereby sphingomyelinase-catalyzed hydrolysis of the substrate into the corresponding 2-alkanoylamino-4-nitrophenol and phosphorylcholine takes place in an amount proportional to the sphingomyelinase activity in the extract. Alkalinization of the 2-alkanoylamino-4-nitrophenol thereby produced to convert it into the alkali salt results in the development of a bright yellow color whose intensity is proportional to the amount of the substrate that has been hydrolyzed. Measurement of the intensity of the yellow color, for example, by means of a simple colorimeter or photometer, provides an accurate indication of the sphingomyelinase activity in the extract.