Intestinal anti-inflammatory activity of combined quercitrin and dietary olive oil supplemented with fish oil, rich in EPA and DHA (n-3) polyunsaturated fatty acids, in rats with DSS-induced colitis
Intestinal anti-inflammatory activity of combined quercitrin and dietary olive oil supplemented with fish oil, rich in EPA and DHA (n-3) polyunsaturated fatty acids, in rats with DSS-induced colitis
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DOI：
10.1016/j.clnu.2005.12.009
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发表时间：
2006-06-01
影响因子：
6.3
通讯作者：
Galvez, J.
中科院分区：
文献类型：
--
作者：
Camuesco, D.;Comalada, M.;Galvez, J.
背景与目的：在实验性大鼠结肠炎模型中，已有研究描述了生物黄酮类QR和富含n-3多不饱和脂肪酸(PUFA)的饮食对大鼠肠道的抗炎作用。方法：雌性Wistar大鼠在饮水中分别加入5%(w/v)和2%(w/v)的葡聚糖硫酸钠(DSS)诱导结肠炎。共5组大鼠(n=10)，其中两组在结肠炎诱导前至实验结束前给予富含n-3多不饱和脂肪酸的橄榄油饲料(FO饲料)2周，另一组在改变DSS浓度时开始每日给予QR(1 mg/kg，PO)。另两组用标准饲料喂养诱导DSS大鼠结肠炎，其中一组给予QR治疗。非结肠炎组喂食标准饮食也包括在内。结果：与未治疗的结肠炎大鼠相比，FO饮食和QR同时给药具有明显的肠道抗炎作用，其结肠炎症的各项生化指标明显改善。因此，与未治疗的结肠炎大鼠相比，结肠髓过氧化物酶(MPO)和碱性磷酸酶(AP)的活性都显著降低。此外，QR加FO饮食治疗的大鼠结肠谷胱甘肽含量完全恢复，这一含量甚至高于单纯FO饮食治疗的大鼠。与对照组相比，联合治疗组大鼠结肠一氧化氮合酶和环氧合酶-2表达降低，白三烯B-4、肿瘤坏死因子α和白细胞介素1β水平显著降低，对结肠炎的抑制作用明显增强。结论：在饮食中加入橄榄油和n-3多不饱和脂肪酸对大鼠肠炎性疾病具有潜在的协同作用。(C)2006年爱思唯尔有限公司和欧洲临床营养与代谢学会。版权所有。
Background & Aims: Previous studies have described the intestinal anti-inflammatory effects exerted by the bioflavonoid quercitrin (QR) and by an n-3 polyunsaturated fatty acids (PUFA)-enriched diet in experimental models of rat colitis. The aim of the present study was to test if the combination of both treatments would result in an improvement in the intestinal anti-inflammatory effect achieved separately.Methods: Colitis was induced in female Wistar rats by incorporating dextran sodium sulfate (DSS) in drinking water at 5% (w/v) for 5 days and at 2% (w/v) for the following 10 days. Five groups of rats (n = 10) were used: two of them received an olive-oil-based diet with fish oil, rich in n-3 PUFA (FO diet) for 2 weeks before colitis induction and until the end of the experiment, and one of those also was administered daily QR (1 mg/kg, PO), starting when DSS concentration was changed. DSS colitis was induced in other two groups fed with standard rat diet, one of them being administered QR as before. A non-colitic group fed standard diet was also included. After that period, the rats were sacrificed and colonic damage was assessed both histologically and biochemically.Results: The concurrent administration of FO diet and QR exhibited an intestinal anti-inflammatory effect, as evidenced by a significant improvement of all biochemical parameters of colonic inflammation assayed in comparison with non-treated colitic rats. Thus, both colonic myeloperoxidase (MPO) and alkaline phosphatase (AP) activities were significantly reduced compared with untreated colitic rats. In addition, a complete restoration of colonic glutathione content, which was depleted as a consequence of the colonic insult, was obtained in rats treated with QR plus FO diet; this content was even higher than that obtained when colitic rats were treated with FO diet alone. When compared with the control colitic group, the combined treatment was also associated with a lower colonic nitric oxide synthase and cyclooxygenase-2 expression as well as with a significant reduction in different colonic proinflammatory mediators assayed, i.e. leukotriene B-4, tumor necrosis factor alpha and interteukin 1 beta, showing a significantly greater inhibitory effect of the tatter in comparison with rats receiving FO diet without the flavonoid.Conclusions: These results support the potential synergism between the administration of the flavonoid and the incorporation of olive oil and n-3 PUFA to the diet for the treatment of these intestinal inflammatory disorders. (C) 2006 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.