Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria
Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria
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掺入 VSV-G 产生融合质膜囊泡，能够有效转移生物活性大分子和线粒体
DOI：
10.1007/s10544-016-0066-y
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发表时间：
2016-05
期刊：
影响因子：
--
通讯作者：
Wei CJ
中科院分区：
文献类型：
--
作者：
Lin HP;Zheng DJ;Li YP;Wang N;Chen SJ;Fu YC;Xu WC;Wei CJ
The objective of this study was to determine if plasma membrane vesicles (PMVs) could be exploited for efficient transfer of macro-biomolecules and mitochondria. PMVs were derived from mechanical extrusion, and made fusogenic (fPMVs) by incorporating the glycoprotein G of vesicular stomatitis virus (VSV-G). Confocal microscopy examination revealed that cytoplasmic proteins and mitochondria were enclosed in PMVs as evidenced by tracing with cytoplasmically localized and mitochondria-targeted EGFP, respectively. However, no fluorescence signal was detected in PMVs from cells whose nucleus was labeled with an EGFP-tagged histone H2B. Consistently, qRT-PCR measurement showed that mRNA, miRNA and mitochondrial DNA decreased slightly; while nuclear DNA was not measureable. Further, Western blot analysis revealed that cytoplasmic and membrane-bound proteins fell inconspicuously while nuclear proteins were barely detecsle. In addition, fPMVs carrying cytoplamic DsRed proteins transduced about ~40 % of recipient cells. The transfer of protein was further confirmed by using the inducible Cre/loxP system. Mitochondria transfer was found in about 20 % recipient cells after incubation with fPMVs for 5 h. To verify the functionalities of transferred mitochondria, mitochodria-deficient HeLa cells (Rho0) were generated and cultivated with fPMVs. Cell enumeration demonstrated that adding fPMVs into culture media stimulated Rho0 cell growth by 100 % as compared to the control. Lastly, MitoTracker and JC-1 staining showed that transferred mitochondria maintained normal shape and membrane potential in Rho0 cells. This study established a time-saving and efficient approach to delivering proteins and mitochondria by using fPMVs, which would be helpful for finding a cure to mitochondria-associated diseases.Graphical abstractSchematic of the delivery of macro-biomolecules and organelles by fPMVs. VSV-G-expressing cells were extruded through a 3 μm polycarbonate membrane filter to generate fusogenic plasma membrane vesicles (fPMVs), which contain bioactive molecules and organelles but not the nucleus. fPMVs can be endocytosed by target cells, while the cargo is released due to low-pH induced membrane fusion. These nucleus-free fPMVs are efficient at delivery of cytoplasmic proteins and mitochondria, leading to recovery of mitochondrial biogenesis and proliferative ability in mitochondria-deficient cells.
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DOI：
10.1016/0016-5085(87)90704-9
发表时间：
1987-06
期刊：
The Journal of surgical research
影响因子：
--
作者：
Michael E Zenilman;Maria Fiani;Philip D. Stahl;Elizabeth M. Brunt;M. Flye
通讯作者：
Michael E Zenilman;Maria Fiani;Philip D. Stahl;Elizabeth M. Brunt;M. Flye
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DOI：
10.1016/j.jconrel.2014.03.053
发表时间：
2014-09-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Anselmo AC;Mitragotri S
通讯作者：
Mitragotri S
影响因子：
4.2
作者：
Patel A;Cholkar K;Mitra AK
通讯作者：
Mitra AK
影响因子：
4.2
作者：
Wei Zhang;G. Gu;Xing Shen;Qi Zhang;Gang Wang;Pei-jun Wang
通讯作者：
Wei Zhang;G. Gu;Xing Shen;Qi Zhang;Gang Wang;Pei-jun Wang
DOI：
10.1073/pnas.1006586107
发表时间：
2010-10-26
影响因子：
11.1
作者：
Du, Heng;Guo, Lan;Yan, Shirley ShiDu
通讯作者：
Yan, Shirley ShiDu