HUPO Plasma Proteome Project specimen collection and handling: Towards the standardization of parameters for plasma proteome samples
HUPO Plasma Proteome Project specimen collection and handling: Towards the standardization of parameters for plasma proteome samples
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DOI：
10.1002/pmic.200401245
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发表时间：
2005-08-01
期刊：
影响因子：
3.4
通讯作者：
Chan, DW
中科院分区：
文献类型：
--
作者：
Rai, AJ;Gelfand, CA;Chan, DW
有大量的分析前变量可以改变血液样本的分析。我们已经就其中一些问题进行了研究，包括样品类型的选择、储存过程中的稳定性、蛋白酶抑制剂的使用以及临床标准化。由于在HuPo PPP工作中有广泛的样品变量和广泛的分析技术，因此不可能为样品或其处理定义单一的分析前标准清单。我们在这里提出了一个观察纲要，借鉴了实际结果和合理的临床理论和实践。基于我们的数据，我们发现：(1)在某些多肽研究中，去血小板血浆比血清更可取；(2)样品应均分并最好储存在液氮中；(3)建议添加蛋白酶抑制剂，但应及早并入并谨慎使用，因为某些形式的非特异性蛋白质加合物和其他形式干扰多肽研究。此外，建议(4)认真跟踪分析前变量和(5)使用标准物质进行质量控制和质量保证。这些发现有助于为样品处理问题提供指导，总体建议是意识到所有可能的分析前变量作为任何蛋白质组学研究的先决条件。
There is a substantial list of pre-analytical variables that can alter the analysis of blood-derived samples. We have undertaken studies on some of these issues including choice of sample type, stability during storage, use of protease inhibitors, and clinical standardization. As there is a wide range of sample variables and a broad spectrum of analytical techniques in the HUPO PPP effort, it is not possible to define a single list of pre-analytical standards for samples or their processing. We present here a compendium of observations, drawing on actual results and sound clinical theories and practices. Based on our data, we find that (1) platelet-depleted plasma is preferable to serum for certain peptidomic studies; (2) samples should be aliquoted and stored preferably in liquid nitrogen; (3) the addition of protease inhibitors is recommended, but should be incorporated early and used judiciously, as some form non specific protein adducts and others interfere with peptide studies. Further, (4) the diligent tracking of pre-analytical variables and (5) the use of reference materials for quality control and quality assurance, are recommended. These findings help provide guidance on sample handling issues, with the overall suggestion being to be conscious of all possible pre-analytical variables as a prerequisite of any proteomic study.