Co-transcription pattern of an introgressed operon in the maize chloroplast genome comprising four ATP synthase subunit genes and the ribosomal rps2
Co-transcription pattern of an introgressed operon in the maize chloroplast genome comprising four ATP synthase subunit genes and the ribosomal rps2
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玉米叶绿体基因组中渗入操纵子的共转录模式，包含四个 ATP 合酶亚基基因和核糖体 rps2
DOI：
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发表时间：
1993
影响因子：
5.1
通讯作者：
A. Subramanian
中科院分区：
文献类型：
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作者：
D. Stahl;S. Rodermel;L. Bogorad;A. Subramanian
当将叶绿体 RP 基因簇与大肠杆菌或蓝细菌中的基因簇进行比较时，发现了将基因从一种基因复合体引入另一种基因复合体（基因渗入）的几个例子。在这里，我们描述了来自玉米（Zea mays）的一个此类簇的转录模式，其中包括类囊体 ATP 合酶的 4 个亚基（atpI、H、F、A）和 rps2 基因的基因。从深色玉米幼苗和绿化玉米幼苗中分离的 RNA 中鉴定出 12 种大小范围为 7 000 至 800 nt 的转录物，并通过逆转录分析对其中几种进行了表征。一个长度为 6 200 nt 的主要物种，其 5' 端位于 rps2 起始 ATG 上游 181 nt 处，包含所有 5 个基因的转录本。另外两组转录物的 5' 末端大约为 5'。还鉴定了 atpI 和 atpH 基因起始密码子上游 120 和 50 nt。因此，玉米中的质体基因簇在功能上被组织为具有额外调节特征的操纵子，以允许增加类囊体成分的mRNA积累。
Several examples of the introduction of a gene from one gene complex into another (introgression) are found when chloroplast RP gene clusters are compared to those in Escherichia coli or cyanobacteria. Here we describe the transcript pattern of one such cluster from maize (Zea mays) that includes the genes for 4 subunits of the thylakoid ATP synthase (atpI, H, F, A) and the rps2 gene. Twelve transcript species covering the size range from 7 000 to 800 nt were identified in RNA isolated from dark-grown and greening maize seedlings, and several of them were characterized by reverse transcription analysis. A major species of 6 200 nt, with its 5′ end at 181 nt upstream of the initiating ATG of rps2, contained the transcripts of all the 5 genes. Two further sets of transcripts having their 5′ ends ca. 120 and 50 nt upstream of the initiation codons of the atpI and atpH genes were also identified. Thus, this plastid gene cluster in maize is functionally organized as an operon with additional regulatory features to allow for increased accumulation of mRNAs for the thylakoid components.