Chicken interferon regulatory factor 1 (IRF1) involved in antiviral innate immunity via regulating IFN-β production
Chicken interferon regulatory factor 1 (IRF1) involved in antiviral innate immunity via regulating IFN-β production
复制标题
鸡干扰素调节因子 1 (IRF1) 通过调节 IFN-β 产生参与抗病毒先天免疫
DOI：
10.1016/j.dci.2018.07.003
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发表时间：
2018-11-01
影响因子：
2.9
通讯作者：
Yan, Yaxian
中科院分区：
文献类型：
--
作者：
Liu, Yunxia;Cheng, Yuqiang;Yan, Yaxian
干扰素调节因子是病毒感染过程中调节干扰素表达的一个重要家族。IRF1、IRF3和IRF7是在哺乳动物中触发I型干扰素反应的主要调节因子。然而，IRF3被认为是哺乳动物中最关键的调节因子，但在鸡中是缺失的，目前还不清楚IRF1是否参与了I型干扰素信号通路的缺失。在这里，我们确定鸡IRF1(ChIRF1)是病毒感染反应中一个关键的干扰素-β介体。过表达chIRF1可强烈激活干扰素-β，抑制禽流感病毒和新城疫病毒的复制。此外，在chIRF1过表达过程中，干扰素-β和ISGs的mRNA水平升高。此外，缺失突变分析表明，chIRF1的前四个结构域是诱导干扰素-β所必需的。综上所述，我们的研究表明chIRF1是干扰素-β的重要调节因子，并参与鸡的抗病毒天然免疫。
Interferon regulatory factors (IRFs) is an important family for IFN expression regulating while viral infection. IRF1, IRF3, and IRF7 are the primary regulators that trigger type I IFN response in mammals. However, IRF3, which has been identified as the most critical regulator in mammals, is absent in chickens, and it is unknown whether IRF1 is involved in type I IFN signaling pathways in IRF3-deficient chicken cells. Here, we identified chicken IRF1 (chIRF1) as a critical IFN-beta mediator in response to viral infection. Overexpression of chIRF1 activated IFN-beta intensively and suppressed AIV and NDV viral replication. Moreover, the mRNA levels of IFN-beta and ISGs increased during chIRF1 overexpression. In addition, deletion mutant analysis revealed that the first four domains of chIRF1 are indispensable for IFN-beta induction. Together, our studies demonstrate that chIRF1 is an important regulator of IFN-beta and is involved in chicken antiviral innate immunity.