ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice
ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice
复制标题
DOI：
10.1016/0014-5793(96)00811-3
复制
发表时间：
1996-08-26
期刊：
影响因子：
3.5
通讯作者：
Narumiya, S
中科院分区：
文献类型：
--
作者：
Nakagawa, O;Fujisawa, K;Narumiya, S
我们最近发现了一种新的人类蛋白激酶，p160 ROCK，作为一个假定的下游目标的小GTdR Rho。以人ROCK cDNA为探针，从小鼠文库中分离出两个不同的高度相关序列的cDNA，一个编码人ROCK的小鼠对应物（ROCK-Ⅰ），另一个编码一个新的ROCK。ROCK相关激酶（ROCK-II）。与ROCK/ROCK-I一样，ROCK-II也选择性地与GTP-Rho结合，ROCK-I mRNA在脑和肌肉中广泛表达，而ROCK-II mRNA在脑、肌肉、心脏、肺和胎盘中大量表达。这些结果表明，在同一物种中至少存在两种ROCK异构体，并且在Rho介导的信号通路中发挥不同的作用。
We recently identified a novel human protein kinase, p160 ROCK, as a putative downstream target of the small GTPase Rho. Using the human ROCK cDNA as a probe, we isolated cDNA of two distinct, highly related sequences from mouse libraries, One encoded a mouse counterpart of human ROCK (ROCK-I), and the other encoded a novel. ROCK-related kinase (ROCK-II). Like ROCK/ROCK-I, ROCK-II also bound to GTP-Rho selectively, ROCK-I mRNA was ubiquitously expressed except in the brain and muscle, whereas ROCK-II mRNA was expressed abundantly in the brain, muscle, heart, lung and placenta, These results suggest that at least two ROCK isoforms are present in a single species and play distinct roles in Rho-mediated signalling pathways.