Distribution of neuraminidase in Arthrobacter and its purification by affinity chromatography.
Distribution of neuraminidase in Arthrobacter and its purification by affinity chromatography.
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神经氨酸酶在节杆菌中的分布及其亲和层析纯化。
DOI：
10.1093/oxfordjournals.jbchem.a131830
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发表时间：
1977
影响因子：
2.7
通讯作者：
T. Sugimori
中科院分区：
文献类型：
--
作者：
Y. Uchida;Y. Tsukada;T. Sugimori
Neuraminidase [sialidase, EC 3.2.1.18] was found to be widely distributed in bacteria belonging to Arthrobacter. Among these bacteria, Arthrobacter ureafaciens, A. oxydans, and A. aurescens produced relatively potent neuraminidase activities. For the production of this enzyme, not only colominic acid, a homopolymer of N-acetylneuraminic acid, but also N-acetylneuraminic acid, the reaction product of this enzyme, are effective as sources of carbon. An affinity adsorbent specific for neuraminidase was prepared by cross-linking colominic acid with soluble starch by means of epichlorohydrin. Neuraminidase from A. ureafaciens could be purified on this affinity column. The purified neuraminidase was shown to be free from protease, N-acetylneuraminic acid aldolase, phospholipase C, and glycosidases. Aminoff's assay procedure for sialic acid was modified to avoid the centrifugation step. The modified procedure gave a higher molecular extinction coefficient.
